GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

It is now the 3rd CHALLENGE Period for CACAO Fall 2019! It will end on Sunday October 20, 2019 at 11:59 pm CDT
This is your chance to challenge other team's annotations. You may also DEFEND or suggest improvements to your own annotations IF they have been challenged. Please note, although we ENCOURAGE challenges, an excess of identical challenges that do not appear to be applicable to the annotation or well thought out will be considered spam and ignored.
Have any questions? Please email us at ecoliwiki@gmail.com

Category:GO:0032991 ! protein-containing complex

Jump to: navigation, search

DAG for GO:0032991id: GO:0032991

name: protein-containing complex
namespace: cellular_component
alt_id: GO:0043234
def: "A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together." [GOC:dos, GOC:mah]
comment: A protein complex in this context is meant as a stable set of interacting proteins which can be co-purified by an acceptable method, and where the complex has been shown to exist as an isolated, functional unit in vivo. Acceptable experimental methods include stringent protein purification followed by detection of protein interaction. The following methods should be considered non-acceptable: simple immunoprecipitation, pull-down experiments from cell extracts without further purification, colocalization and 2-hybrid screening. Interactions that should not be captured as protein complexes include: 1) enzyme/substrate, receptor/ligand or any similar transient interactions, unless these are a critical part of the complex assembly or are required e.g. for the receptor to be functional; 2) proteins associated in a pull-down/co-immunoprecipitation assay with no functional link or any evidence that this is a defined biological entity rather than a loose-affinity complex; 3) any complex where the only evidence is based on genetic interaction data; 4) partial complexes, where some subunits (e.g. transmembrane ones) cannot be expressed as recombinant proteins and are excluded from experiments (in this case, independent evidence is necessary to find out the composition of the full complex, if known). Interactions that may be captured as protein complexes include: 1) enzyme/substrate or receptor/ligand if the complex can only assemble and become functional in the presence of both classes of subunits; 2) complexes where one of the members has not been shown to be physically linked to the other(s), but is a homologue of, and has the same functionality as, a protein that has been experimentally demonstrated to form a complex with the other member(s); 3) complexes whose existence is accepted based on localization and pharmacological studies, but for which experimental evidence is not yet available for the complex as a whole.
subset: goslim_agr
subset: goslim_chembl
subset: goslim_flybase_ribbon
subset: goslim_generic
subset: goslim_pir
synonym: "macromolecular complex" EXACT []
synonym: "macromolecule complex" EXACT []
synonym: "protein complex" NARROW []
synonym: "protein containing complex" EXACT []
synonym: "protein-protein complex" NARROW []
is_a: GO:0005575 ! cellular_component

AmiGO <GOterm>GO:0032991</GOterm>
Gene Ontology Home

The contents of this box are automatically generated. You can help by adding information to the "Notes"

Usage Notes


See Help:References for how to manage references in GONUTS.

Child Terms

This category has the following 200 subcategories, out of 468 total.

(previous page) (next page)

Pages in category "GO:0032991 ! protein-containing complex"

The following 200 pages are in this category, out of 614 total.

(previous page) (next page)
Jump to pages starting with: B C D E F H M P R S X Y