BPT4:TOPS

Species (Taxon ID)	Enterobacteria phage T4 (Bacteriophage T4). (10665)
Gene Name(s)	60
Protein Name(s)	DNA topoisomerase small subunit Protein Gp60
External Links
UniProt	P23992
EMBL	M19728 AF158101 X52686
PIR	JT0209
RefSeq	NP_049618.1
PDB	5NP6
PDBsum	5NP6
ProteinModelPortal	P23992
SMR	P23992
GeneID	1258779
KEGG	vg:1258779
Proteomes	UP000009087
GO	GO:0005524 GO:0003677 GO:0003918 GO:0006265
Gene3D	3.40.50.670
InterPro	IPR001241 IPR013760 IPR013759 IPR031660
PANTHER	PTHR10169
Pfam	PF16898
SUPFAM	SSF56719

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0009330	DNA topoisomerase complex (ATP-hydrolyzing)	PMID:6296073	ECO:0000315	mutant phenotype evidence used in manual assertion		C		In figure 7, gp60 was replaced with a mutant version, amE429 or amE594, and then the protein complex was eluted and run through a gel. Lanes 1-3 are gels of increasingly concentrated fractions of amE492 infected cells. Lane 4 uses the same fraction as lane 3, but eluted with .1M potassium phosphate buffer, as a control for the buffer. Lane 5 is the same fraction as lane 3, but eluted with .3M potassium phosphate buffer. Lane 6 is the same as lane 5, but with .5M potassium phosphate buffer. Lane 6 has the standards for the three proteins in topoisomerase, p39, p52, and p60. Lanes 8 and 9 are the same as lanes 5 and 6, respectively, but using amE594 as the mutant gene 60 instead of amE429. In lanes 5 and 8, p60 is absent (due to the mutation) and p39 is also absent. P52 is evident, meaning it was able to be eluted by the .3M buffer. In lanes 6 and 9, p60 is again absent as is p52, since it had already been eluted out. P39 is evident, as it was eluted out of fraction by .5M buffer. Since both proteins were eluted out by different concentrations of buffer, they were not bound together, which differs from past research that proves them bound together in complex. Therefore, p60 must be capable of binding them together without any conformational change of p39 or p52(both proteins' identities were confirmed after experiment).	complete CACAO 13106
part_of	GO:0032991	protein-containing complex	PMID:226976	ECO:0000314	direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
contributes_to	GO:0003916	DNA topoisomerase activity	PMID:226976	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR001241 InterPro:IPR013759	F		Seeded From UniProt	complete
enables	GO:0003918	DNA topoisomerase type II (ATP-hydrolyzing) activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR001241 InterPro:IPR013759 InterPro:IPR013760	F		Seeded From UniProt	complete
enables	GO:0005524	ATP binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR001241 InterPro:IPR013759 InterPro:IPR013760	F		Seeded From UniProt	complete
involved_in	GO:0006265	DNA topological change	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR001241 InterPro:IPR013759	P		Seeded From UniProt	complete
enables	GO:0005524	ATP binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0067	F		Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0238	F		Seeded From UniProt	complete
enables	GO:0000166	nucleotide binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0547	F		Seeded From UniProt	complete
enables	GO:0003916	DNA topoisomerase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0799	F		Seeded From UniProt	complete
enables	GO:0016853	isomerase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0413	F		Seeded From UniProt	complete
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References

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