| requireschanges | HAEDU:HGBA | 2012-09-23 17:25:21 CDT | GO:0030492 hemoglobin binding (F) | PMID:7890379 | ECO:0000314 direct assay evidence used in manual assertion | Fig 1 shows SDS-page gel of HgbA from solubilized outer membranes of H. ducreyi strain 35000 affinity purified on human hemoglobin.
| challenge |
| acceptable | HAEDC:Q9K2H6 | 2012-09-23 17:32:09 CDT | GO:0009279 cell outer membrane (C) | PMID:10678980 | ECO:0000314 direct assay evidence used in manual assertion | Fig 5 shows the SDS-PAGE and Western blot identifying DsrA. Figure caption includes details about preparation of outer membrane for experiment.
| challenge |
| acceptable | HAEDC:F6KZW9 | 2012-10-16 19:13:12 CDT | GO:0030682 evasion or tolerance of host defense response (P) | PMID:21444663 | ECO:0000315 mutant phenotype evidence used in manual assertion | Figures 5 and 6 show the effect of mtrC deletion on resistance to the mammalian defenses LL-37 and beta defensin. The plasmid-complemented mutant was not significantly different from the parent.
| challenge |
| acceptable | HAEDC:F6KZW9 | 2012-10-16 13:47:10 CDT | GO:0010628 positive regulation of gene expression (P) | PMID:21444663 | ECO:0000315 mutant phenotype evidence used in manual assertion | Figure 4 shows the effect of mtrC deletion on mRNA levels of four CpxRA-regulated genes. The genes fimA and omp2B are known to be upregulated by CpxRA, while lspB and dsrA are downregulated by activation of the CpxRA regulation system. These results, combined with the information in Figure 3C, led the authors to conclude that the CpxRA system was activated in the mtrC deletion mutant.
| challenge |
| acceptable | AGGAC:Q7DK12 | 2012-10-16 19:12:13 CDT | GO:0044072 negative regulation by symbiont of host cell cycle (P) | PMID:10946289 | ECO:0000315 mutant phenotype evidence used in manual assertion | Figures 4 and 5 show the effect of targeted modifications of cdt genes on immunoinhibition. Human T cells were incubated with proteins produced from the cdt mutant-carrying plasmids (schematic in figure 3), and the cell cycle distribution was analyzed. Of particular interest are Figures 5bc, which demonstrate the high levels of G2 arrest in the cdtB mutant.
| challenge |
| requireschanges | HAEDU:CDTA | 2012-10-16 18:13:18 CDT | GO:0001907 killing by symbiont of host cells (P) | PMID:11453636 | ECO:0000314 direct assay evidence used in manual assertion | Figure 2D shows that the eluate of a cdtA mutant strain is not detectably cytotoxic. Cytotoxicity can be restored by mixture of CdtB and CdtC with the sonicated cell lysate of E. coli expressing cdtA. This recovery was not seen upon combination of CdtB and CdtC alone or with the sonicated cell lysate of E.coli carrying a control plasmid (Figure 4, especially 4G vs 4FH).
| challenge |
| acceptable | HAEDC:Q67BF6 | 2012-10-17 19:06:32 CDT | GO:0009405 pathogenesis (P) | PMID:16428791 | ECO:0000315 mutant phenotype evidence used in manual assertion | Table 1 shows the effect of inoculation of human volunteers with H. ducreyi. Subjects were infected with the wild-type on one arm and the DltA mutant on the other. The mutant had decreased papule formation, demonstrating that DltA plays a role in pathogenesis.
| challenge |
| requireschanges | HAEDU:G1UBA5 | 2012-10-17 21:16:17 CDT | GO:0009279 cell outer membrane (C) | PMID:11401984 | ECO:0000315 mutant phenotype evidence used in manual assertion | Fig 3 shows the SDS-PAGE and Western blot identifying D15. Figure caption includes details about preparation of outer membrane for experiment. Figure 2 shows comparison of the amino acid sequence to other D15 proteins.
| challenge |
| requireschanges | HAEDC:Q9ZHL3 | 2012-10-17 20:31:08 CDT | GO:0020012 evasion or tolerance of host immune response (P) | PMID:14500520 | ECO:0000315 mutant phenotype evidence used in manual assertion | Figures 1 and 2 show the ability of the LspA1 mutant to inhibit the phagocytic activity of HL-60 and U-937, respectively. The LspA1 mutant, shown in column 3, is very like the wild type (column 1).
| challenge |
| requireschanges | HAEDC:Q9ZHL0 | 2012-10-17 20:31:30 CDT | GO:0052200 response to host defenses (P) | PMID:14500520 | ECO:0000315 mutant phenotype evidence used in manual assertion | Figures 1 and 2 show the ability of the LspA2 mutant to inhibit the phagocytic activity of HL-60 and U-937, respectively. The LspA2 mutant, shown in column 4, is very like the wild type (column 1).
| challenge |
| acceptable | HAEDU:Q7VLH6 | 2012-10-17 21:52:21 CDT | GO:0010629 negative regulation of gene expression (P) | PMID:19451237 | ECO:0000315 mutant phenotype evidence used in manual assertion | Figure 5 shows increased expression of LspA2, LspA1, and LspB in CpxR mutants (lane 2 vs lane 2) and decreased expression in complemented mutants (lane 3 vs lane 4), indicating negative regulation of these genes by CpxR.
| challenge |
| acceptable | HAEDU:Q7VM01 | 2012-10-18 10:54:45 CDT | GO:0030682 evasion or tolerance of host defense response (P) | PMID:20086092 | ECO:0000315 mutant phenotype evidence used in manual assertion | Figure 3 shows the effect of sapA mutation on resistance to the mammalian defense LL-37. The plasmid-complemented mutant was not significantly different from the parent. No resistance was seen for alpha- and beta- defensins.
| challenge |
| acceptable | HAEDC:Q9K2H6 | 2012-10-18 14:09:29 CDT | GO:0009405 pathogenesis (P) | PMID:11179317 | ECO:0000315 mutant phenotype evidence used in manual assertion | Tables 1 and 2 show the results of inoculation with wild-type and DsrA mutant H. ducreyi. Table 1 shows that the mutant is impaired in its ability to cause papules. Table 2 shows that the mutant (at an increased dose) forms smaller papules than the wild-type, on par with the heat-inactivated wild-type.
| challenge |
| acceptable | HAEDC:Q9K2H6 | 2012-10-18 14:33:44 CDT | GO:0020012 evasion or tolerance of host immune response (P) | PMID:10678980 | ECO:0000315 mutant phenotype evidence used in manual assertion | Figure 6 shows that the DsrA mutant has lower resistance to bactericidal killing than the wild-type organism. Figure 7 shows that complementation of the mutant restores resistance
| challenge |
| acceptable | HAEDC:Q58ZK4 | 2012-10-18 16:20:09 CDT | GO:0009279 cell outer membrane (C) | PMID:16622201 | ECO:0000314 direct assay evidence used in manual assertion | The materials and methods section entitled "Radioimmunoprecipitation of H. ducreyi outer membrane proteins" describes the process used to isolate the outer membrane proteins.
| challenge |
| acceptable | HAEDC:Q58ZK4 | 2012-10-18 16:20:09 CDT | GO:0009405 pathogenesis (P) | PMID:16622201 | ECO:0000315 mutant phenotype evidence used in manual assertion | Tables 1 and 2 show the results of inoculation of wild-type and ncaA mutant H. ducreyi. The mutant had decreased virulence in the swine (table 1) and human (table 2) models of infection compared to the wild-type.
| challenge |
