PMID:19451237

Citation	Labandeira-Rey, M, Mock, JR and Hansen, EJ (2009) Regulation of expression of the Haemophilus ducreyi LspB and LspA2 proteins by CpxR. Infect. Immun. 77:3402-11
Abstract	The LspA1, LspA2, and LspB proteins of Haemophilus ducreyi comprise a two-partner secretion system that has been shown to be necessary for H. ducreyi to inhibit phagocytosis by immune cells in vitro. Inactivation of lspA1 resulted in increased levels of LspA2, suggesting that these two proteins are differentially controlled (C. J. Ward et al., Infect. Immun. 71:2478-2486, 2003). Expression of LspA2 but not LspA1 was shown to be both growth phase dependent and affected by the presence of fetal calf serum (FCS) in the growth medium. In addition, neither LspA1 nor LspA2 could be detected in culture supernatant fluid in the absence of FCS. DNA microarray analysis revealed that 324 H. ducreyi genes were differentially regulated after growth in the presence of FCS. Among these, the CpxRA two-component sensory transduction system was downregulated by the presence of FCS. Inactivation of cpxR resulted in increased expression of both LspB and LspA2. Electrophoretic mobility shift assays showed that a recombinant H. ducreyi CpxR protein bound the promoter region of the lspB-lspA2 operon. The cpxR and cpxA genes were shown to be part of an operon containing two additional genes in H. ducreyi 35000HP. This is the first description of a two-component sensory transduction system regulating a proven virulence factor of H. ducreyi.
Links	PubMed PMC2715676 Online version:10.1128/IAI.00292-09
Keywords	Bacterial Outer Membrane Proteins/biosynthesis; Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Bacterial Proteins/physiology; DNA, Bacterial/metabolism; Electrophoretic Mobility Shift Assay; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Gene Knockout Techniques; Haemophilus ducreyi/physiology; Humans; Lectins/biosynthesis; Oligonucleotide Array Sequence Analysis; Promoter Regions, Genetic; Protein Binding; Signal Transduction
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HAEDU:Q7VLH6		GO:0010629: negative regulation of gene expression	ECO:0000315:		P		Figure 5 shows increased expression of LspA2, LspA1, and LspB in CpxR mutants (lane 2 vs lane 2) and decreased expression in complemented mutants (lane 3 vs lane 4), indicating negative regulation of these genes by CpxR.	complete CACAO 5355
HAEDU:Q7VLH6	involved_in	GO:0010629: negative regulation of gene expression	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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