BPPHS:E

Species (Taxon ID)	Enterobacteria phage phiX174 (Isolate Sanger) (Bacteriophagephi-X174). (1217068)
Gene Name(s)	E
Protein Name(s)	Lysis protein E Protein E GPE
External Links
UniProt	P03639
EMBL	J02482 Z35638
PIR	A04247
RefSeq	NP_040709.1
ProteinModelPortal	P03639
TCDB	9.B.6.1.1
GeneID	2546400
Proteomes	UP000005893
GO	GO:0033644 GO:0016021 GO:0004857 GO:0044659 GO:0019054
InterPro	IPR007605
Pfam	PF04517
ProDom	PD012101

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0044659	cytolysis by virus of host cell	PMID:10760296	ECO:0000314			P		Figure 2A: phage E protein lyses E. coli cells expressing wildtype slyD and mraY - absorbance decreases over time	complete CACAO 10541
	GO:0019077	lytic viral release	PMID:22742425	ECO:0000315			P		Figure 2A	complete CACAO 5438
	GO:0039635	suppression by virus of host peptidoglycan biosynthetic process	PMID:10760296	ECO:0000316		UniProtKB:P0A6W3	P		Figure 2D: more time was required for phage E protein to lyse cells in E. coli expressing additional mraY (produced by plasmids, represented by square) than in E. coli expressing normal amounts of mraY (represented by circle) - shows that E targets mraY - mraY is known to catalyze a reaction involved in peptidoglycan synthesis (reference 39)	complete CACAO 10546
	GO:0039640	cytolysis by virus via suppression of host peptidoglycan biosynthetic process	PMID:10760296	ECO:0000316		UniProtKB:P0A6W3	P		Fig 2D – E-mediated lysis occurs in wt host cells (circle), is delayed in cells overexpressing MraY (MraY expressed from medium copy plasmid, square), and doesn’t occur in cells overexpressing an MraY mutant (MraY mutant expressed from same medium copy plasmid, triangle). Suggests that E causes host lysis by inhibiting MraY. MraY is an enzyme involved in PG biosynthesis.	complete CACAO 11831
	GO:0039635	suppression by virus of host peptidoglycan biosynthetic process	PMID:11078734	ECO:0000314			P		Fig 3 – E expression inhibits [3H]-DAP incorporation into the cell wall (black square = E expression, black circle = no E expression) Suggests that E inhibits cell wall biosynthesis – DAP is an amino acid unique to the bacterial cell wall Fig 4B – E expression inhibits [3H]-DAP incorporation into PG biosynthesis lipid intermediates (black bar = no E expression, grey bar = E expression) Suggests that E inhibits PG biosynthesis specifically – PG biosynthesis consists of three stages: nucleotide intermediate synthesis, lipid intermediate synthesis, and addition of PG precursors from lipid intermediates to actual PG – lipid intermediate synthesis is inhibited by E expression	complete CACAO 11872
involved_in	GO:0039640	cytolysis by virus via suppression of host peptidoglycan biosynthetic process	PMID:11078734	ECO:0000314	direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
	GO:0039640	cytolysis by virus via suppression of host peptidoglycan biosynthetic process	PMID:11078734	ECO:0000314			P		E causes host lysis: Fig 3 – E expression causes decrease in cell density indicative of host cell lysis (clear square = E expression, clear circle = no E expression) E inhibits PG biosynthesis: Fig 3 – E expression inhibits [3H]-DAP incorporation into the cell wall is significantly inhibited by expression of E (black square = E expression, black circle = no E expression) Suggests that E inhibits cell wall biosynthesis – DAP is an amino acid unique to the bacterial cell wall Fig 4B – E expression inhibits [3H]-DAP incorporation into PG biosynthesis lipid intermediates is significantly inhibited by expression of E (black bar = no E expression, grey bar = E expression) Suggests that E inhibits PG biosynthesis specifically – PG biosynthesis consists of three stages: nucleotide intermediate synthesis, lipid intermediate synthesis, and addition of PG precursors from lipid intermediates to actual PG – lipid intermediate synthesis is inhibited by E expression	complete CACAO 11873
involved_in	GO:0039635	suppression by virus of host peptidoglycan biosynthetic process	PMID:10760296	ECO:0000316	genetic interaction evidence used in manual assertion	UniProtKB:P0A6W3	P		Seeded From UniProt	complete
involved_in	GO:0044659	cytolysis by virus of host cell	PMID:10760296	ECO:0000314	direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0044659	cytolysis by virus of host cell	PMID:22742425	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0043086	negative regulation of catalytic activity	GO_REF:0000108	ECO:0000366	evidence based on logical inference from automatic annotation used in automatic assertion	GO:0004857	P		Seeded From UniProt	complete
enables	GO:0004857	enzyme inhibitor activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR007605	F		Seeded From UniProt	complete
involved_in	GO:0019054	modulation by virus of host process	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR007605	P		Seeded From UniProt	complete
part_of	GO:0033644	host cell membrane	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1043	C		Seeded From UniProt	complete
part_of	GO:0016021	integral component of membrane	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0812	C		Seeded From UniProt	complete
part_of	GO:0016020	membrane	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0472	C		Seeded From UniProt	complete
involved_in	GO:0016032	viral process	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0945	P		Seeded From UniProt	complete
involved_in	GO:0019835	cytolysis	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0204	P		Seeded From UniProt	complete
part_of	GO:0020002	host cell plasma membrane	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1032 UniProtKB-SubCell:SL-0375	C		Seeded From UniProt	complete
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References

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