User:Theresa.Hubbell

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	DEIRA:Q9RS96	2013-03-28 13:08:36 CDT	GO:0047429 nucleoside-triphosphate diphosphatase activity (F)	PMID:3162430	ECO:0000314 direct assay evidence used in manual assertion	Figure 6	challenge
updatedbyinstructor	DEIRA:Q9RS96	2013-03-28 13:22:45 CDT	GO:0047429 nucleoside-triphosphate diphosphatase activity (F)	PMID:21733847	ECO:0000314 direct assay evidence used in manual assertion	Figure 6 shows the enzyme activity of DR2231	challenge

acceptable:1
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Theresa.Hubbell

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

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My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
unacceptable	MOUSE:PLIN5	2012-10-07 15:04:00 CDT	GO:0010890 positive regulation of sequestering of triglyceride (P)	PMID:22675471	ECO:0000314 direct assay evidence used in manual assertion	Figure #3 shows that "a higher concentration of TGs was observed in cells overexpressing LSDP5 compared with controls".	challenge
unacceptable	MOUSE:PLIN5	2012-11-03 21:01:59 CDT	GO:0010890 positive regulation of sequestering of triglyceride (P)	PMID:3365886	ECO:0000314 direct assay evidence used in manual assertion	Figure 3b says that "lipid droplets were stained using BODIPY (neutral lipid dye). BODIPY fluorescence increased in cells overexpressing LSDP5. The mRNA level of adipophilin, a protein that coats lipid droplets, was increased in cells overexpressing LSDP5 (Figure 3C). The amount of TGs in cells overexpressing LSDP5 also significantly increased, compared to control cells, which was determined using a TG test kit (P=0.014) (Figure 3D). The phenotypes of increased lipid droplet storage and increased TG contents were also observed in primary hepatocytes (Figure S1). These data demonstrate that the overexpression of LSDP5 is associated with an increased cellular TG content".	challenge
unacceptable	MOUSE:PLIN1	2012-11-03 21:26:35 CDT	GO:0045598 regulation of fat cell differentiation (P)	PMID:2853465	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3 shows that "Body weight gain of female hTg and mTg mice was similar to WT controls when fed a normal chow diet (ND) (data not shown). In contrast, when female mice were fed a HFD, both hTg and mTg mice gained weight less rapidly than their WT littermates (Fig. 3A, B). Male hTg mice had a very small but significant reduction in body weight gain when fed a HFD compared with WT littermates, whereas mTg did not differ from WT in their weight gain on an HFD. Since both female hTg and mTg mice demonstrated a more robust phenotype compared with male transgenic mice, the data presented throughout this manuscript focused only on female mice. At 30 weeks of age (25 weeks of HFD), both hTg and mTg mice weighed ~20% less than WT controls (Fig. 3A, B). The observed difference in body weight became significant after the animals reached 13 (hTg) and 22 (mTg) weeks of age, respectively. Regardless of diet, food intake was similar between Tg and WT mice and was not a contributing factor to the observed differences in body weight on the HFD (WT 2.28 ± 0.16g vs. 2.09 ± 0.09g, P = 0.55). In spite of their similar food consumption level, fasting serum leptin concentration was significantly lower in HFD-fed hTg mice compared with WT mice (9.83 ± 3.57 vs. 32.85 ± 0.02 ng/ml) (P = 0.003), which is probably explained by smaller adipocyte in hTg mice."	challenge
updatedbyinstructor	MOUSE:CAV1	2012-11-18 11:46:38 CST	GO:0060546 negative regulation of necroptosis (P)	PMID:23049990	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 5 shows that "fibrosis and cell death characterises adipose tissue from CAV1 -/- mice".	challenge
requireschanges	MOUSE:PLIN2	2012-11-18 11:56:37 CST	GO:0010885 regulation of cholesterol storage (P)	PMID:22427949	ECO:0000314 direct assay evidence used in manual assertion	Figure 3 shows that "Perilipin 2 deficiency does not increase cholesterol-induced toxicity in macrophages".	challenge

acceptable:0
unacceptable:3
requires_changes:1
flagged:0

Annotations challenged by Theresa.Hubbell

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
requireschanges	Ellie.detrich, Team Triple Bond	RAT:LITAF	GO:0050663 - cytokine secretion (P)	PMID:17475886	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 2
requireschanges	Ellie.detrich, Team Triple Bond	MOUSE:GPNMB	GO:0032695 - negative regulation of interleukin-12 production (P)	PMID:17475886	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1
requireschanges	Cbice1, Team Smarter Who?	VIBFI:Q9ANY0	GO:0075294 - positive regulation by symbiont of entry into host (P)	PMID:19182778	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1
requireschanges	Cbice1, Team Smarter Who?	ORYSJ:Q67J23	GO:0080113 - regulation of seed growth (P)	PMID:22209874	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1
updatedbyinstructor	Sarahtheriault, Team Gene Simmons	HUMAN:CATS	GO:0006508 - proteolysis (P)	PMID:22864553	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 2

0 annotations fixed by Theresa.Hubbell