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PMID:7890379

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Citation

Elkins, C (1995) Identification and purification of a conserved heme-regulated hemoglobin-binding outer membrane protein from Haemophilus ducreyi. Infect. Immun. 63:1241-5

Abstract

A hemoglobin-binding protein (HgbA) from Haemophilus ducreyi was identified and purified. The 100-kDa HgbA was detected in all strains of H. ducreyi tested, and a somewhat larger hemoglobin-binding protein was found in one strain of Haemophilus influenzae. HgbA was purified and the amino acid sequence of the N terminus of HgbA revealed no significant homologies with known proteins. Two different antisera to HgbA from H. ducreyi 35000 recognized HgbA proteins from all tested H. ducreyi strains; they did not recognize proteins from the H. influenzae strain. Expression of HgbA was regulated by the level of heme but not by iron present in the medium. Animal species of hemoglobin competed with iodinated human hemoglobin for binding to whole cells of H. ducreyi and supported the growth of H. ducreyi. The lack of immunological cross-reactivity and the differences in hemoglobin specificities between the H. ducreyi and the H. influenzae hemoglobin-binding proteins suggest that they are unrelated.

Links

PubMed PMC173141

Keywords

Amino Acid Sequence; Bacterial Outer Membrane Proteins/isolation & purification; Bacterial Outer Membrane Proteins/metabolism; Bacterial Proteins; Carrier Proteins/isolation & purification; Carrier Proteins/metabolism; Cross Reactions; Haemophilus ducreyi/chemistry; Haemophilus ducreyi/metabolism; Heme/pharmacology; Hemoglobins/metabolism; Molecular Sequence Data; Species Specificity

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HAEDU:HGBA

GO:0030492: hemoglobin binding

ECO:0000314:

F

Fig 1 shows SDS-page gel of HgbA from solubilized outer membranes of H. ducreyi strain 35000 affinity purified on human hemoglobin.

complete
CACAO 5146


See also

References

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