PMID:10829075

Citation	Dahlqvist, A, Stahl, U, Lenman, M, Banas, A, Lee, M, Sandager, L, Ronne, H and Stymne, S (2000) Phospholipid:diacylglycerol acyltransferase: an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants. Proc. Natl. Acad. Sci. U.S.A. 97:6487-92
Abstract	Triacylglycerol (TAG) is known to be synthesized in a reaction that uses acyl-CoA as acyl donor and diacylglycerol (DAG) as acceptor, and which is catalyzed by the enzyme acyl-CoA:diacylglycerol acyltransferase. We have found that some plants and yeast also have an acyl-CoA-independent mechanism for TAG synthesis, which uses phospholipids as acyl donors and DAG as acceptor. This reaction is catalyzed by an enzyme that we call phospholipid:diacylglycerol acyltransferase, or PDAT. PDAT was characterized in microsomal preparations from three different oil seeds: sunflower, castor bean, and Crepis palaestina. We found that the specificity of the enzyme for the acyl group in the phospholipid varies between these species. Thus, C. palaestina PDAT preferentially incorporates vernoloyl groups into TAG, whereas PDAT from castor bean incorporates both ricinoleoyl and vernoloyl groups. We further found that PDAT activity also is present in yeast microsomes. The substrate specificity of this PDAT depends on the head group of the acyl donor, the acyl group transferred, and the acyl chains of the acceptor DAG. The gene encoding the enzyme was identified. The encoded PDAT protein is related to lecithin:cholesterol acyltransferase, which catalyzes the acyl-CoA-independent synthesis of cholesterol esters. However, budding yeast PDAT and its relatives in fission yeast and Arabidopsis form a distinct branch within this protein superfamily, indicating that a separate PDAT enzyme arose at an early point in evolution.
Links	PubMed PMC18631 Online version:10.1073/pnas.120067297
Keywords	Acyl Coenzyme A/physiology; Acyltransferases/genetics; Acyltransferases/metabolism; Catalysis; Diacylglycerol O-Acyltransferase; Microsomes/metabolism; Plants/metabolism; Saccharomyces cerevisiae/metabolism; Sterol O-Acyltransferase/metabolism; Substrate Specificity; Triglycerides/biosynthesis
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
YEAST:PDAT		GO:0046027: phospholipid:diacylglycerol acyltransferase activity	ECO:0000266:	UniProtKB:P16301	F		Figure 5	complete CACAO 5273
YEAST:PDAT		GO:0046027: phospholipid:diacylglycerol acyltransferase activity	ECO:0000315:		F		Figure 2	complete CACAO 5326
YEAST:PDAT	enables	GO:0046027: phospholipid:diacylglycerol acyltransferase activity	ECO:0000315: mutant phenotype evidence used in manual assertion		F		Seeded From UniProt	complete

References

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