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User:Priesko4

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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableHAEI8:MURA2013-03-26 11:18:57 CDTGO:0008760 UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity (F)PMID:23462005ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 demonstrates the inhibitory activities on MurA and then determines the residual activity.

Figure 5 demonstrates the inhibitory actions of sulfhydryl group dependent activity on the mutant phenotype.

challenge
updatedbyinstructorRHILE:Q20DQ42013-04-09 12:24:21 CDTGO:0008378 galactosyltransferase activity (F)PMID:22110131ECO:0000314 direct assay evidence used in manual assertion

Figure 3 demonstrates the rgtA activity through its ability to be a galA donor and viewing the total lipid extracts from strains with the enzyme vs. strains without the enzyme

challenge
acceptableSALTY:Q9XDN02013-04-09 12:36:43 CDTGO:0004022 alcohol dehydrogenase (NAD) activity (F)PMID:23077559ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 demonstrates the ability for the mutant to grow on media with minimal or saturated B12.

Figure 6 shows that PduQ regenerates NAD+ for the PduP reaction in purified Pdu MCPs by demonstrating the concentration of propionyl-CoA in comparison with a mutant phenotype.

challenge
updatedbyinstructorARTAU:Q6SJY72013-04-10 12:31:41 CDTGO:0004040 amidase activity (F)PMID:16885454ECO:0000314 direct assay evidence used in manual assertion

Figure 5 demonstrates the activity of Zn(II) and Co(II) trzN with selected s-triazines as substrates.

Figure 4 shows the effect of metal addition on ametryn hydrolysis activity of native TrzN purified from recombinant cells grown on 500M zinc and metal-depleted TrzN.

challenge
updatedbyinstructorLEPIR:C0J1Q32013-04-10 12:55:48 CDTGO:0045916 negative regulation of complement activation (P)PMID:22911815ECO:0000314 direct assay evidence used in manual assertion

Figure 3 shows the inhibition of both the alternative and classical pathways of complement activation through the inhibition of complement-mediated hemolysis of rabbit erthrocytes.

challenge

acceptable:2
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Priesko4

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
updatedbyinstructorPriesko4,
Team ttKtt
ARTAU:Q6SJY7GO:0004040 - amidase activity (F)PMID:16885454ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2

fixed by Priesko4
updatedbyinstructorReddenc1,
Team Better Than Hot Pocket
XANC5:Q3BUB5GO:0043709 - cell adhesion involved in single-species biofilm formation (P)PMID:21995764ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 6
updatedbyinstructorPallasbr,
Team KEGGstands at PubMed
HUMAN:E2F1GO:0045893 - positive regulation of transcription, DNA-templated (P)PMID:22476863ECO:0000314 direct assay evidence used in manual assertionchallengeC: 3
updatedbyinstructorZdebstep,
Team Hot Pockets
ECOLX:E2QE90GO:0002949 - tRNA threonylcarbamoyladenosine modification (P)PMID:21183954ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 4

fixed by Zdebstep
updatedbyinstructorAcoppola,
Team CFP
HUMAN:NLGN2GO:0032024 - positive regulation of insulin secretion (P)PMID:22528485ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1
updatedbyinstructorSinghpr4,
Team The Monstars
CLOPH:NIGPGO:0004645 - 1,4-alpha-oligoglucan phosphorylase activity (F)PMID:21808968ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2

fixed by Priesko4
requireschangesAmanda.karpa,
Team Cytoskeleton
MOUSE:ITSN1GO:0043524 - negative regulation of neuron apoptotic process (P)PMID:23054079ECO:0000314 direct assay evidence used in manual assertionchallengeC: 4
unacceptableElkington,
Team Old Worm
CAEEL:Q9GZG4GO:1900369 - negative regulation of post-transcriptional gene silencing by RNA (Figure 4A)PMID:17110334IMP: Inferred from Mutant Phenotype challengeC: 3
updatedbyinstructorMill2034,
Team Its Miller Time
ECOLI:CPXRGO:0045893 - positive regulation of transcription, DNA-templated (P)PMID:15743952ECO:0000314 direct assay evidence used in manual assertionchallengeC: 3
updatedbyinstructorMill2034,
Team Its Miller Time
ARATH:AB1DGO:0016042 - lipid catabolic process (P)PMID:12065405ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 4
updatedbyinstructorPallasbr,
Team KEGGstands at PubMed
MOUSE:FOSL2GO:0045893 - positive regulation of transcription, DNA-templated (P)PMID:22585681ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2
updatedbyinstructorVthompsonui,
Team Buster McThunderstick
MYCTU:PATGO:0016746 - acyltransferase activity (F)PMID:21627103ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1

fixed by Priesko4
requireschangesVthompsonui,
Team Buster McThunderstick
MYCTU:ACSAGO:0006476 - protein deacetylation (P)PMID:21627103ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
unacceptableOptimus Prime,
Team Cybertron
ARATH:DAPA2GO:0008840 - 4-hydroxy-tetrahydrodipicolinate synthase activity (F)PMID:22792278ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2
updatedbyinstructorBrianna.diehl,
Team Plasma Membrane
MOUSE:NDRG1GO:0008285 - negative regulation of cell population proliferation (P)PMID:23555679ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 4
updatedbyinstructorMill1856,
Team Its Miller Time
NOSS1:Q8Z0A3GO:0030611 - arsenate reductase activity (F)PMID:23086594ECO:0000316 genetic interaction evidence used in manual assertionchallengeC: 2

3 annotations fixed by Priesko4

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