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User:Mill1856

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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableGEOSL:Q74D232013-04-10 23:38:02 CDTGO:0009297 pilus assembly (P)PMID:15973408ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 shows that pilA deficient mutants are unable to produce pili compared to the wild-type, and that complementation of the mutant strain with a functional copy of pilA restores function.

challenge
updatedbyinstructorNOSS1:Q8Z0A32013-04-12 13:44:59 CDTGO:0030611 arsenate reductase activity (F)PMID:23086594ECO:0000316 genetic interaction evidence used in manual assertion

Figure 6 shows a complementation assay of arsenate reductase activity in ΔarsC E. coli WC3110. When all0195, an arsenate reductase from Anabaena sp. PCC7120, is transformed into E. coli that has its arsenate reducatase gene (arsC) deleted, it regains function and it able to perform similar to the wild-type strain.

challenge
updatedbyinstructorPSEAE:FUR2013-04-14 12:56:39 CDTGO:0019290 siderophore biosynthetic process (P)PMID:8478325ECO:0000315 mutant phenotype evidence used in manual assertion

Table 3 shows that the fur mutant constitutively produces pyoverdin. When the mutant is complemented with the wild-type gene it no longer constitutively produces pyoverdin.

challenge
acceptableALLVD:DSRE2013-04-14 14:39:53 CDTGO:0019417 sulfur oxidation (P)PMID:18952098ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 shows that dsrE mutant is unable to oxidize sulfur to sulfate. A complementation with the wild-type dsrE gene restores the ability of A. vinosum to oxidize sulfur to sulfate.

challenge
updatedbyinstructorCHLTE:Q8KAB92013-04-14 14:54:29 CDTGO:0019418 sulfide oxidation (P)PMID:21233162ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4 shows that the dsrM mutant is unable to oxidize sulfur globules to sulfate like the wild-type. When a complementation is done with the wild-type dsrM gene, ability to oxidize sulfur globules to sulfate is restored.

challenge
acceptableGEOSL:Q747G22013-04-14 17:12:43 CDTGO:0033215 iron assimilation by reduction and transport (P)PMID:16000176ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4 shows that the OmpJ mutant is unable to reduce Fe(III) like the wild-type.

challenge
unacceptableFRATU:K0E8562013-04-14 17:16:25 CDTGO:0009297 pilus assembly (Figure 3 shows that pilF mutants do not produce pili like the wild-type. When complemented with a plasmid containing the functional pilF gene, the mutant regains the ability to produce pili.)PMID:18426883IMP: Inferred from Mutant Phenotype
challenge

acceptable:3
unacceptable:1
requires_changes:0
flagged:0

Annotations challenged by Mill1856

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
unacceptableRomero.alan,
Team BTHO PubMed!
PSEPK:TOLBGO:0051301 - cell division (P)PMID:10940016ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1
updatedbyinstructorMkelly,
Team CFP
DANRE:Q20CG4GO:0036135 - Schwann cell migration (P)PMID:21965611ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2

fixed by Mill1856
updatedbyinstructorVthompsonui,
Team Buster McThunderstick
MOUSE:UPP1GO:0006218 - uridine catabolic process (P)PMID:23355744ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2

fixed by Mill1856
updatedbyinstructorMill1856,
Team Its Miller Time
CHLTE:Q8KAB9GO:0019418 - sulfide oxidation (P)PMID:21233162ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2

2 annotations fixed by Mill1856

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