User:Singhpr4

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	PSEAE:PCHA	2013-03-28 23:21:23 CDT	GO:0042864 pyochelin biosynthetic process (P)	PMID:12624097	ECO:0000314 direct assay evidence used in manual assertion	Figure 4 represents in vitro study to determine rate limiting factor in Salicylate production. The figure shows that Isochorismate synthase is the rate limiting enzyme in production of Salicylate, which is an intermediate for Pyochelin biosynthesis.	challenge
updatedbyinstructor	BRASZ:NAAB	2013-04-07 18:12:28 CDT	GO:0051213 dioxygenase activity (F)	PMID:21498645	ECO:0000314 direct assay evidence used in manual assertion	Fig 3 shows biotransformation of 5-NSA and 5-CSA by E.coli cell extract containing Naab (having dioxygenase activity) from Bradyrhizobium.	challenge
updatedbyinstructor	BRASZ:NAAC	2013-04-07 18:14:56 CDT	GO:0046573 lactonohydrolase activity (F)	PMID:21498645	ECO:0000314 direct assay evidence used in manual assertion	Fig 4 shows biotransformation of lactones by E. coli cell extract containing NaaC (having lactone hydrolase activity) from Bradyrhizobium.	challenge
requireschanges	9GAMM:C8YX85	2013-04-07 18:24:00 CDT	GO:1902087 dimethylsulfoniopropionate catabolic process (P)	PMID:19807777	ECO:0000314 direct assay evidence used in manual assertion	Figure 4B shows DMSP catabolic activity of dddD. E. coli containing plasmid pBIO 1733 which has dddD genes can catabolize DMSP to produce 3HP detected by HPLC as chromatogram peak. (direct assay).	challenge
updatedbyinstructor	PSEAE:PHHC	2013-04-07 18:36:21 CDT	GO:0006572 tyrosine catabolic process (P)	PMID:9846749	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 5 illustrates the inability of a PhhC-inactivated mutant to utilize L-tyrosine as a sole carbon source. Therefore, a mutant with inactive PhhC can not degrade L-tyrosine.	challenge
acceptable	PSEAE:Q9HZV9	2013-04-07 18:38:59 CDT	GO:0047925 geranoyl-CoA carboxylase activity (F)	PMID:18469096	ECO:0000314 direct assay evidence used in manual assertion	Fig 3B demonstrates geranoyl coenzyme A carboxylase activity with Cis-geranyl-COA as substrate. This step is involved in catabolic pathway of acyclic terpenes.	challenge
updatedbyinstructor	CLOPH:NIGP	2013-04-07 18:43:10 CDT	GO:0004645 phosphorylase activity (F)	PMID:21808968	ECO:0000314 direct assay evidence used in manual assertion	Fig 2 shows direct assay result confirming phosphorolytic reaction (or glycosyltransferase activity) of nigerose phosphorylase	challenge
updatedbyinstructor	PSEKB:CLCE	2013-04-07 18:47:43 CDT	GO:0018506 maleylacetate reductase activity (F)	PMID:8407778	ECO:0000314 direct assay evidence used in manual assertion	Figure 2 shows chloroaromatics degradation by Maleylacetate Reductase in Pseudomonas sp. strain B13.	challenge
acceptable	CUPPJ:TFDF2	2013-04-07 18:49:45 CDT	GO:1901168 3-chlorocatechol catabolic process (P)	PMID:12107121	ECO:0000314 direct assay evidence used in manual assertion	Fig 3 shows direct assay of Maleyiacetate reductase 2 (enzyme required for growth of Ralstonia eutropha on chloroaromatics) activity in Ralstonia eutropha JMP222 which a derivative of strain JMP134, cured of plasmid pJP4. The study is done using anion exchange chromatography showing peaks of enzyme activity.	challenge
acceptable	CUPPJ:TFDF1	2013-04-07 18:51:57 CDT	GO:1901168 3-chlorocatechol catabolic process (P)	PMID:12107121	ECO:0000314 direct assay evidence used in manual assertion	Fig 3 shows direct assay of Maleyiacetate reductase 1 (enzyme required for growth of Ralstonia eutropha on chloroaromatics) activity in Ralstonia eutropha JMP222 which is a derivative of strain JMP134, cured of plasmid pJP4. The study is done using anion exchange chromatography showing peaks of enzyme activity.	challenge
acceptable	CUPPJ:P94138	2013-04-07 18:54:03 CDT	GO:1901168 3-chlorocatechol catabolic process (P)	PMID:12107121	ECO:0000314 direct assay evidence used in manual assertion	Fig 3 shows direct assay of Chloromuconate cycloisomerase(enzyme required for growth of Ralstonia eutropha on chloroaromatics) activity in Ralstonia eutropha JMP222 which is a derivative of strain JMP134, cured of plasmid pJP4. The study is done using anion exchange chromatography showing peaks of enzyme activity.	challenge
correctedthroughchallenges	9SPHN:G0ZNC1	2013-04-07 19:04:00 CDT	GO:0052764 exo-oligoalginate lyase activity (F)	other:www.sciencedirect.com/science/article/pii/S1226086X11001651	ECO:0000314 direct assay evidence used in manual assertion	Fig 3 shows alginate monosaccharide to be the degradation products of alginate by the recombinant MJ-3 oligoalginate lyase.	challenge
updatedbyinstructor	SPHPI:Q8RQW0	2013-04-10 20:40:24 CDT	GO:0019617 protocatechuate catabolic process, meta-cleavage (P)	PMID:12486039	ECO:0000314 direct assay evidence used in manual assertion	Figure 2 shows 4-carboxy-4-hydroxy-2-oxoadipate aldolase (LigK) catalyzes break down of CHA (intermediate product of protocatechuate cleavage) into pyruvate and oxaloacetate.	challenge
acceptable	ORYSJ:CYNS	2013-04-10 21:34:44 CDT	GO:0008824 cyanate hydratase activity (F)	PMID:21494323	ECO:0000314 direct assay evidence used in manual assertion	Fig 3 shows cyanase activity of OsCYN at different pH and temperatures.	challenge
updatedbyinstructor	BURCE:ANDAB	2013-04-12 22:44:12 CDT	GO:0043421 anthranilate catabolic process (P)	PMID:13129960	ECO:0000314 direct assay evidence used in manual assertion	Fig 3 shows Anthranilate 1,2-dioxygenase ferredoxin subunit is necessary (along with 2 other subunits) to oxidize anthranilate to catechol. Without Anthranilate 1,2-dioxygenase ferredoxin subunit, anthranilate degradation is not possible in Burkholderia cepacia.	challenge
updatedbyinstructor	BURCE:Q9KJH7	2013-04-14 12:57:36 CDT	GO:0016491 oxidoreductase activity (F)	PMID:10795678	ECO:0000314 direct assay evidence used in manual assertion	Tabe 2 shows preference of 2,4,5-Trihydroxytoluene as substrate for trihydroxytoluene dioxygenase.	challenge

acceptable:6
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Singhpr4

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Singhpr4

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