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PMID:21494323

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Citation

Qian, D, Jiang, L, Lu, L, Wei, C and Li, Y (2011) Biochemical and structural properties of cyanases from Arabidopsis thaliana and Oryza sativa. PLoS ONE 6:e18300

Abstract

Cyanate is toxic to all organisms. Cyanase converts cyanate to CO₂ and NH₃ in a bicarbonate-dependent reaction. The biophysical functions and biochemical characteristics of plant cyanases are poorly studied, although it has been investigated in a variety of proteobacteria, cyanobacteria and fungi. In this study, we characterised plant cyanases from Arabidopsis thaliana and Oryza sativa (AtCYN and OsCYN). Prokaryotic-expressed AtCYN and OsCYN both showed cyanase activity in vitro. Temperature had a similar influence on the activity of both cyanases, but pH had a differential impact on AtCYN and OsCYN activity. Homology modelling provided models of monomers of AtCYN and OsCYN, and a coimmunoprecipitation assay and gel filtration indicated that AtCYN and OsCYN formed homodecamers. The analysis of single-residue mutants of AtCYN indicated that the conserved catalytic residues also contributed to the stability of the homodecamer. KCNO treatment inhibited Arabidopsis germination and early seedling growth. Plants containing AtCYN or OsCYN exhibited resistance to KCNO stress, which demonstrated that one role of cyanases in plants is detoxification. Transcription level of AtCYN was higher in the flower than in other organs of Arabidopsis. AtCYN transcription was not significantly affected by KCNO treatment in Arabidopsis, but was induced by salt stress. This research broadens our knowledge on plant detoxification of cyanate via cyanase.

Links

PubMed PMC3070753 Online version:10.1371/journal.pone.0018300

Keywords

Amino Acid Sequence; Arabidopsis/enzymology; Carbon-Nitrogen Lyases/chemistry; Carbon-Nitrogen Lyases/metabolism; Gene Expression Regulation, Plant; Immunoprecipitation; Molecular Sequence Data; Oryza sativa/enzymology; Plant Proteins/chemistry; Plant Proteins/metabolism; Sequence Homology, Amino Acid; Temperature

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:CYNS

acts_upstream_of_or_within

GO:0009651: response to salt stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:CYNS

acts_upstream_of_or_within

GO:0009440: cyanate catabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ORYSJ:CYNS

GO:0008824: cyanate hydratase activity

ECO:0000314:

F

Fig 3 shows cyanase activity of OsCYN at different pH and temperatures.

complete
CACAO 7597

ORYSJ:CYNS

enables

GO:0008824: cyanate hydratase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:CYNS

enables

GO:0008824: cyanate hydratase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:CYNS

involved_in

GO:0009440: cyanate catabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:CYNS

involved_in

GO:0009651: response to salt stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:CYNS

enables

GO:0042802: identical protein binding

ECO:0000353: physical interaction evidence used in manual assertion

AGI_LocusCode:AT3G23490

F

Seeded From UniProt

complete


See also

References

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