PMID:13129960

Citation	Chang, HK, Mohseni, P and Zylstra, GJ (2003) Characterization and regulation of the genes for a novel anthranilate 1,2-dioxygenase from Burkholderia cepacia DBO1. J. Bacteriol. 185:5871-81
Abstract	Anthranilate (2-aminobenzoate) is an important intermediate in tryptophan metabolism. In order to investigate the degradation of tryptophan through anthranilate by Burkholderia cepacia, several plasposon mutations were constructed of strain DBO1 and one mutant with the plasposon insertion in the anthranilate dioxygenase (AntDO) genes was chosen for further study. The gene sequence obtained from flanking DNA of the plasposon insertion site revealed unexpected information. B. cepacia DBO1 AntDO (designated AntDO-3C) is a three-component Rieske-type [2Fe-2S] dioxygenase composed of a reductase (AndAa), a ferredoxin (AndAb), and a two-subunit oxygenase (AndAcAd). This is in contrast to the two-component (an oxygenase and a reductase) AntDO enzyme from Acinetobacter sp. strain ADP1, P. aeruginosa PAO1, and P. putida P111. AntDO from strains ADP1, PAO1, and P111 are closely related to benzoate dioxygenase, while AntDO-3C is closely related to aromatic hydrocarbon dioxygenases from Novosphingobium aromaticivorans F199 and Sphingomonas yanoikuyae B1 and 2-chlorobenzoate dioxygenase from P. aeruginosa strains 142 and JB2. Escherichia coli cells expressing the functional AntDO-3C genes transform anthranilate and salicylate (but not 2-chlorobenzoate) to catechol. The enzyme includes a novel reductase whose absence results in less efficient transformation of anthranilate by the oxygenase and ferredoxin. AndR, a possible AraC/XylS-type transcriptional regulator, was shown to positively regulate expression of the andAcAdAbAa genes. Anthranilate was the only effector (of 12 aromatic compounds tested) that was able to induce expression of the genes.
Links	PubMed PMC193950
Keywords	Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Burkholderia cepacia/enzymology; Burkholderia cepacia/genetics; Cloning, Molecular; Gene Expression Regulation, Bacterial; Mixed Function Oxygenases/classification; Mixed Function Oxygenases/genetics; Mixed Function Oxygenases/metabolism; Molecular Sequence Data; Mutation; Restriction Mapping; Sequence Analysis, DNA; Substrate Specificity; Tryptophan/metabolism; ortho-Aminobenzoates/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BURCE:ANDAB		GO:0043421: anthranilate catabolic process	ECO:0000314:		P		Fig 3 shows Anthranilate 1,2-dioxygenase ferredoxin subunit is necessary (along with 2 other subunits) to oxidize anthranilate to catechol. Without Anthranilate 1,2-dioxygenase ferredoxin subunit, anthranilate degradation is not possible in Burkholderia cepacia.	complete CACAO 7770
BURCE:ANDAB	involved_in	GO:0043421: anthranilate catabolic process	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

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