PMID:12107121

Citation	Plumeier, I, Pérez-Pantoja, D, Heim, S, González, B and Pieper, DH (2002) Importance of different tfd genes for degradation of chloroaromatics by Ralstonia eutropha JMP134. J. Bacteriol. 184:4054-64
Abstract	The tfdC(I)D(I)E(I)F(I,) and tfdD(II)C(II)E(II)F(II) gene modules of plasmid pJP4 of Ralstonia eutropha JMP134 encode complete sets of functional enzymes for the transformation of chlorocatechols into 3-oxoadipate, which are all expressed during growth on 2,4-dichlorophenoxyacetate (2,4-D). However, activity of tfd(I)-encoded enzymes was usually higher than that of tfd(II)-encoded enzymes, both in the wild-type strain grown on 2,4-D and in 3-chlorobenzoate-grown derivatives harboring only one tfd gene module. The tfdD(II)-encoded chloromuconate cycloisomerase exhibited special kinetic properties, with high activity against 3-chloromuconate and poor activity against 2-chloromuconate and unsubstituted muconate, thus explaining the different phenotypic behaviors of R. eutropha strains containing different tfd gene modules. The enzyme catalyzes the formation of an equilibrium between 2-chloromuconate and 5-chloro- and 2-chloromuconolactone and very inefficiently catalyzes dehalogenation to form trans-dienelactone as the major product, thus differing from all (chloro)muconate cycloisomerases described thus far.
Links	PubMed PMC135226
Keywords	2,4-Dichlorophenoxyacetic Acid/metabolism; Adipates/metabolism; Carboxylic Ester Hydrolases/genetics; Carboxylic Ester Hydrolases/metabolism; Catechols/metabolism; Chlorobenzoates/metabolism; Culture Media; Cupriavidus necator/enzymology; Cupriavidus necator/genetics; Dioxygenases; Gene Expression Regulation, Bacterial; Genes, Bacterial; Intramolecular Lyases/genetics; Intramolecular Lyases/metabolism; Mixed Function Oxygenases/genetics; Mixed Function Oxygenases/metabolism; Oxidoreductases/genetics; Oxidoreductases/metabolism; Oxidoreductases Acting on CH-CH Group Donors; Oxygenases/genetics; Oxygenases/metabolism; Plasmids; Sorbic Acid/analogs & derivatives; Sorbic Acid/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
CUPPJ:P94138		GO:1901168: 3-chlorocatechol catabolic process	ECO:0000314:		P		Fig 3 shows direct assay of Chloromuconate cycloisomerase(enzyme required for growth of Ralstonia eutropha on chloroaromatics) activity in Ralstonia eutropha JMP222 which is a derivative of strain JMP134, cured of plasmid pJP4. The study is done using anion exchange chromatography showing peaks of enzyme activity.	complete CACAO 7346
CUPPJ:P94138	involved_in	GO:1901168: 3-chlorocatechol catabolic process	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
CUPPJ:TFDF1		GO:1901168: 3-chlorocatechol catabolic process	ECO:0000314:		P		Fig 3 shows direct assay of Maleyiacetate reductase 1 (enzyme required for growth of Ralstonia eutropha on chloroaromatics) activity in Ralstonia eutropha JMP222 which is a derivative of strain JMP134, cured of plasmid pJP4. The study is done using anion exchange chromatography showing peaks of enzyme activity.	complete CACAO 7345
CUPPJ:TFDF1	involved_in	GO:1901168: 3-chlorocatechol catabolic process	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
CUPPJ:TFDF2		GO:1901168: 3-chlorocatechol catabolic process	ECO:0000314:		P		Fig 3 shows direct assay of Maleyiacetate reductase 2 (enzyme required for growth of Ralstonia eutropha on chloroaromatics) activity in Ralstonia eutropha JMP222 which a derivative of strain JMP134, cured of plasmid pJP4. The study is done using anion exchange chromatography showing peaks of enzyme activity.	complete CACAO 7344
CUPPJ:TFDF2	involved_in	GO:1901168: 3-chlorocatechol catabolic process	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

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