GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

Category:Team The Monstars

From GONUTS
Jump to: navigation, search
StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorBURCE:Q9KJH7Singhpr4, Team The Monstars2013-04-14 12:57:36 CDTGO:0016491 oxidoreductase activity (F)PMID:10795678ECO:0000314 direct assay evidence used in manual assertion

Tabe 2 shows preference of 2,4,5-Trihydroxytoluene as substrate for trihydroxytoluene dioxygenase.

challenge
unacceptableHUMAN:KDM1ADuquefeg, Team The Monstars2013-04-13 00:56:28 CDTGO:0036208 negative regulation of histone gene expression (P)PMID:23386436ECO:0000270 expression pattern evidence used in manual assertion

Figure. 2 Shows that MAOIs such as tranylcypromine (TCP) were originally designed to target the MAO-A/B. While these compounds also inhibit LSD1. compound OG-L002, which is highly specific to LSD1 (IC50, ~0.02 µM), potently inhibited the expression of viral IE (ICP27 and ICP4) (genesmRNAs were quantitated by quantitative reverse transcription-PCR (qRT-PCR)). The inhibition of LSD1 results in the inactivity of this enzyme, resulting in the repression of HSV-1 IE gene expression. Therefore suggesting that its original activity was to down regulate the IE gene expression

challenge
unacceptableHUMAN:KDM1ADuquefeg, Team The Monstars2013-04-13 00:34:39 CDTGO:0003713 transcription coactivator activity (F)PMID:23386436ECO:0000314 direct assay evidence used in manual assertion

Figure. 2 Shows that MAOIs such as tranylcypromine (TCP) were originally designed to target the MAO-A/B. While these compounds also inhibit LSD1. compound OG-L002, which is highly specific to LSD1 (IC50, ~0.02 µM), potently inhibited the expression of viral IE (ICP27 and ICP4) (genesmRNAs were quantitated by quantitative reverse transcription-PCR (qRT-PCR)). LSD1 shows to be an important co-activator for HCF-1 complex. The inhibition of LSD1 results in the inactivity of this enzyme.

challenge
updatedbyinstructorBURCE:ANDABSinghpr4, Team The Monstars2013-04-12 22:44:12 CDTGO:0043421 anthranilate catabolic process (P)PMID:13129960ECO:0000314 direct assay evidence used in manual assertion

Fig 3 shows Anthranilate 1,2-dioxygenase ferredoxin subunit is necessary (along with 2 other subunits) to oxidize anthranilate to catechol. Without Anthranilate 1,2-dioxygenase ferredoxin subunit, anthranilate degradation is not possible in Burkholderia cepacia.

challenge
unacceptablePSEF5:Q4KFF4Duquefeg, Team The Monstars2013-04-11 10:12:10 CDTGO:0003677 DNA binding (F)PMID:18776018ECO:0000304 author statement supported by traceable reference used in manual assertion

Figure 1. indicated the presence of conserved Anr binding sites in the promoter regions of uspL, uspM, and uspO.

challenge
acceptableORYSJ:CYNSSinghpr4, Team The Monstars2013-04-10 21:34:44 CDTGO:0008824 cyanate hydratase activity (F)PMID:21494323ECO:0000314 direct assay evidence used in manual assertion

Fig 3 shows cyanase activity of OsCYN at different pH and temperatures.

challenge
updatedbyinstructorSPHPI:Q8RQW0Singhpr4, Team The Monstars2013-04-10 20:40:24 CDTGO:0019617 protocatechuate catabolic process, meta-cleavage (P)PMID:12486039ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows 4-carboxy-4-hydroxy-2-oxoadipate aldolase (LigK) catalyzes break down of CHA (intermediate product of protocatechuate cleavage) into pyruvate and oxaloacetate.

challenge
correctedthroughchallenges?Duquefeg, Team The Monstars2013-04-09 21:35:43 CDTGO:0042325 regulation of phosphorylation (Figure 6 (a)(b)(c)(d) Together suggest that Atr works upstream of Chk1regulating its phosphorylation after UV damage.)PMID:10859164IDA: Inferred from Direct Assay
challenge
correctedthroughchallenges9SPHN:G0ZNC1Singhpr4, Team The Monstars2013-04-07 19:04:00 CDTGO:0052764 exo-oligoalginate lyase activity (F)other:www.sciencedirect.com/science/article/pii/S1226086X11001651ECO:0000314 direct assay evidence used in manual assertion

Fig 3 shows alginate monosaccharide to be the degradation products of alginate by the recombinant MJ-3 oligoalginate lyase.

challenge
acceptableCUPPJ:P94138Singhpr4, Team The Monstars2013-04-07 18:54:03 CDTGO:1901168 3-chlorocatechol catabolic process (P)PMID:12107121ECO:0000314 direct assay evidence used in manual assertion

Fig 3 shows direct assay of Chloromuconate cycloisomerase(enzyme required for growth of Ralstonia eutropha on chloroaromatics) activity in Ralstonia eutropha JMP222 which is a derivative of strain JMP134, cured of plasmid pJP4. The study is done using anion exchange chromatography showing peaks of enzyme activity.

challenge
acceptableCUPPJ:TFDF1Singhpr4, Team The Monstars2013-04-07 18:51:57 CDTGO:1901168 3-chlorocatechol catabolic process (P)PMID:12107121ECO:0000314 direct assay evidence used in manual assertion

Fig 3 shows direct assay of Maleyiacetate reductase 1 (enzyme required for growth of Ralstonia eutropha on chloroaromatics) activity in Ralstonia eutropha JMP222 which is a derivative of strain JMP134, cured of plasmid pJP4. The study is done using anion exchange chromatography showing peaks of enzyme activity.

challenge
acceptableCUPPJ:TFDF2Singhpr4, Team The Monstars2013-04-07 18:49:45 CDTGO:1901168 3-chlorocatechol catabolic process (P)PMID:12107121ECO:0000314 direct assay evidence used in manual assertion

Fig 3 shows direct assay of Maleyiacetate reductase 2 (enzyme required for growth of Ralstonia eutropha on chloroaromatics) activity in Ralstonia eutropha JMP222 which a derivative of strain JMP134, cured of plasmid pJP4. The study is done using anion exchange chromatography showing peaks of enzyme activity.

challenge
updatedbyinstructorPSEKB:CLCESinghpr4, Team The Monstars2013-04-07 18:47:43 CDTGO:0018506 maleylacetate reductase activity (F)PMID:8407778ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows chloroaromatics degradation by Maleylacetate Reductase in Pseudomonas sp. strain B13.

challenge
updatedbyinstructorCLOPH:NIGPSinghpr4, Team The Monstars2013-04-07 18:43:10 CDTGO:0004645 phosphorylase activity (F)PMID:21808968ECO:0000314 direct assay evidence used in manual assertion

Fig 2 shows direct assay result confirming phosphorolytic reaction (or glycosyltransferase activity) of nigerose phosphorylase

challenge
acceptablePSEAE:Q9HZV9Singhpr4, Team The Monstars2013-04-07 18:38:59 CDTGO:0047925 geranoyl-CoA carboxylase activity (F)PMID:18469096ECO:0000314 direct assay evidence used in manual assertion

Fig 3B demonstrates geranoyl coenzyme A carboxylase activity with Cis-geranyl-COA as substrate. This step is involved in catabolic pathway of acyclic terpenes.

challenge
updatedbyinstructorPSEAE:PHHCSinghpr4, Team The Monstars2013-04-07 18:36:21 CDTGO:0006572 tyrosine catabolic process (P)PMID:9846749ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 5 illustrates the inability of a PhhC-inactivated mutant to utilize L-tyrosine as a sole carbon source. Therefore, a mutant with inactive PhhC can not degrade L-tyrosine.

challenge
requireschanges9GAMM:C8YX85Singhpr4, Team The Monstars2013-04-07 18:24:00 CDTGO:1902087 dimethylsulfoniopropionate catabolic process (P)PMID:19807777ECO:0000314 direct assay evidence used in manual assertion

Figure 4B shows DMSP catabolic activity of dddD. E. coli containing plasmid pBIO 1733 which has dddD genes can catabolize DMSP to produce 3HP detected by HPLC as chromatogram peak. (direct assay).

challenge
updatedbyinstructorBRASZ:NAACSinghpr4, Team The Monstars2013-04-07 18:14:56 CDTGO:0046573 lactonohydrolase activity (F)PMID:21498645ECO:0000314 direct assay evidence used in manual assertion

Fig 4 shows biotransformation of lactones by E. coli cell extract containing NaaC (having lactone hydrolase activity) from Bradyrhizobium.

challenge
updatedbyinstructorBRASZ:NAABSinghpr4, Team The Monstars2013-04-07 18:12:28 CDTGO:0051213 dioxygenase activity (F)PMID:21498645ECO:0000314 direct assay evidence used in manual assertion

Fig 3 shows biotransformation of 5-NSA and 5-CSA by E.coli cell extract containing Naab (having dioxygenase activity) from Bradyrhizobium.

challenge
acceptablePSEAE:PCHASinghpr4, Team The Monstars2013-03-28 23:21:23 CDTGO:0042864 pyochelin biosynthetic process (P)PMID:12624097ECO:0000314 direct assay evidence used in manual assertion

Figure 4 represents in vitro study to determine rate limiting factor in Salicylate production. The figure shows that Isochorismate synthase is the rate limiting enzyme in production of Salicylate, which is an intermediate for Pyochelin biosynthesis.

challenge

What do the icons mean in the status column?

assessment icons.jpg

Pages in category "Team The Monstars"

The following 2 pages are in this category, out of 2 total.


Jump to pages starting with: D S