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Category:Team Better Than Hot Pocket
|Status||Page||User||Date/Time||GO Term (Aspect)||Reference||Evidence||Notes||Links|
|MOUSE:SIR4||Reddenc1, Team Better Than Hot Pocket||2013-04-13 17:05:49 CDT||GO:0000820 regulation of glutamine family amino acid metabolic process (P)||PMID:23562301||ECO:0000315 mutant phenotype evidence used in manual assertion|
Figure 5) SIR4 knock out cells grew significantly faster than wild type cells. Over expression of SIR4 cells were significantly inhibited in their growth as compared to wild type cells.
|ACIBA:Q2VSW5||Ferran12, Team Better Than Hot Pocket||2013-04-12 00:05:45 CDT||GO:0009405 pathogenesis (P)||PMID:23453128||ECO:0000315 mutant phenotype evidence used in manual assertion|
Mutant, bfms::km, had less biofilm formation than the wildtype, 17978, as seen in figure 2A. Thus supporting that bfms is important for virulence.
|?||Reddenc1, Team Better Than Hot Pocket||2013-04-11 22:11:01 CDT||GO:0010389 regulation of G2/M transition of mitotic cell cycle ((Figure 4.H,I) H3f3b knockouts had a 40% increase in G2/M phase cells compared to the wild type. G0/G1 phase was decreased from 54% to 42%. This was measured with flow cytometry for DNA content.)||PMID:23570311||IMP: Inferred from Mutant Phenotype||challenge|
|ECOLI:MENI||Reddenc1, Team Better Than Hot Pocket||2013-04-11 21:27:32 CDT||GO:0009234 menaquinone biosynthetic process (P)||PMID:23564174||ECO:0000315 mutant phenotype evidence used in manual assertion|
(Figure 4a) ydiI mutants produced 67% less menaquinone-8 than the parent strain.
|MOUSE:UBQL1||Reddenc1, Team Better Than Hot Pocket||2013-04-11 20:22:55 CDT||GO:0034140 negative regulation of toll-like receptor 3 signaling pathway (P)||PMID:21695056||ECO:0000315 mutant phenotype evidence used in manual assertion|
(Figure 1C)The lab group performed a series of reporter assays and found that PLIC-1 is a potent inhibitor of the TLR3-Trif pathway. Over expression of mouse PLIC-1 inhibited Trif-dependent activation of the NFk-B dependent Reporter.
|ARATH:CHLM||Ferran12, Team Better Than Hot Pocket||2013-04-11 13:30:38 CDT||GO:0046406 magnesium protoporphyrin IX methyltransferase activity (F)||PMID:17135235||ECO:0000315 mutant phenotype evidence used in manual assertion|
In figure 2C, methyl transferase activity was observed as florescence of Mg protoporphyrin IX methyl ester was observed in the wildtype CHLM but not in the mutant chlm. The presence of chlorophyll was also observed in the wildtype CHLM,represented by CHLM fluorescences at 630nm, but not in the mutant chlm,figure 2B.
|MOUSE:Q14BW0||Ferran12, Team Better Than Hot Pocket||2013-04-10 22:50:26 CDT||GO:0050881 musculoskeletal movement (P)||PMID:23565223||ECO:0000315 mutant phenotype evidence used in manual assertion|
When compared with wildtype GPR55 mice, GPR55 knock out mice showed a greater chance of falling, figure 4B, and more walking errors,figure 4C.
|STRCO:Q9ADG1||Ferran12, Team Better Than Hot Pocket||2013-04-10 17:32:54 CDT||GO:0016002 sulfite reductase activity (F)||PMID:22659143||ECO:0000315 mutant phenotype evidence used in manual assertion|
In figure 5, mutant N34 with a deletion in SirA and missing SCO6102 failed to grow in nitrite supplemented media, as seen in B, but grew in supplemented sulfur media, as seen in D.
|MOUSE:HIPK1||Reddenc1, Team Better Than Hot Pocket||2013-04-09 13:04:19 CDT||GO:0034333 adherens junction assembly (P)||PMID:23565059||ECO:0000315 mutant phenotype evidence used in manual assertion|
Figure 1(I-J') shows that HIPK1 -/- mice showed a reduced size and density of adherens junctions as compared to the control group.
|XANC5:Q3BUB5||Reddenc1, Team Better Than Hot Pocket||2013-04-09 12:22:03 CDT||GO:0043709 cell adhesion involved in single-species biofilm formation (P)||PMID:21995764||ECO:0000315 mutant phenotype evidence used in manual assertion|
rpfC and rpfG make up a two component system that is responsible for the perception and transduction a signal molecule . Figure 1 shows that rpfG is involved in the enhancement of Xanthomona citri virulence. Mutations rpfG showed a reduction in the virulence of X. citri as measured by canker lesions on the Duncan Grapefruit leaf.
|ARATH:CHLM||Ferran12, Team Better Than Hot Pocket||2013-03-28 19:23:44 CDT||GO:0015995 chlorophyll biosynthetic process (P)||PMID:17135235||ECO:0000315 mutant phenotype evidence used in manual assertion|
In figure 2(a-b) CHLM (wt) showed higher absorbances at approximately 450nm and 650nm with a higher florescence at 630 nm, demonstrating chlorophyll was present in the wildtype and not in the mutant. In c, magnesium protoporphyrin IX methyltransferase is needed to add a methyl onto magnesium protoporphyrin IX (reactant) since a build up of this reactant was seen in the mutant.