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PMID:22659143

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Citation

Fischer, M, Schmidt, C, Falke, D and Sawers, RG (2012) Terminal reduction reactions of nitrate and sulfate assimilation in Streptomyces coelicolor A3(2): identification of genes encoding nitrite and sulfite reductases. Res. Microbiol. 163:340-8

Abstract

The model actinobacterium Streptomyces coelicolor A3(2) uses nitrate and sulfate as nitrogen and sulfur sources, respectively. The final step prior to assimilation into amino acids is the 6-electron reduction of the nitrite and sulfite anions, catalyzed by siroheme-dependent nitrite (NirBD) and sulfite (SirA) reductases. There are two predicted nitrite/sulfite reductases annotated in the genome of S. coelicolor, but it is unclear which is responsible for nitrite and which for sulfite reduction. Here we demonstrate that a knock-out in the genes SCO2487 and SCO2488 encoding NirBD prevents use of nitrite as a nitrogen source, while a knock-out in SCO6102 encoding SirA prevents sulfate assimilation. Both mutations could be phenotypically complemented by supplementation of the growth medium with ammonium or casamino acids in the case of the nirBD mutants or sulfur-containing amino acids in the case of the sirA mutants. No functional redundancy between the genes was observed and we demonstrate that NirBD is exclusively required for assimilatory nitrite (it does not detoxify nitrite) and SirA exclusively for assimilatory sulfite reduction.

Links

PubMed Online version:10.1016/j.resmic.2012.05.004

Keywords

Culture Media/chemistry; Electron Transport; Gene Knockout Techniques; Nitrite Reductases/genetics; Nitrite Reductases/metabolism; Nitrites/metabolism; Oxidation-Reduction; Oxidoreductases Acting on Sulfur Group Donors/genetics; Oxidoreductases Acting on Sulfur Group Donors/metabolism; Streptomyces coelicolor/enzymology; Streptomyces coelicolor/genetics; Streptomyces coelicolor/metabolism; Sulfates/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

STRCO:Q9ADG1

enables

GO:0016002: sulfite reductase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

STRCO:Q9ADG1

GO:0016002: sulfite reductase activity

ECO:0000315:

F

In figure 5, mutant N34 with a deletion in SirA and missing SCO6102 failed to grow in nitrite supplemented media, as seen in B, but grew in supplemented sulfur media, as seen in D.

complete
CACAO 7571


See also

References

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