GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
|Status||Page||User||Date/Time||GO Term (Aspect)||Reference||Evidence||Notes||Links|
|SERPL:Q8KKF5||Kulhan11, Team ttKtt||2013-04-12 19:35:02 CDT||GO:0008843 endochitinase activity (F)||PMID:11734885||ECO:0000314 direct assay evidence used in manual assertion|
Figure 3 depicts the optimum temperature and pH for endochitinase activity.
|METFE:G3P||Kulhan11, Team ttKtt||2013-04-12 19:05:05 CDT||GO:0047100 glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity (F)||PMID:10491162||ECO:0000315 mutant phenotype evidence used in manual assertion|
Table 2 depicts kinetic parameters of both the wild-type and mutant M. fervidus GraP-DHs.
|LEPIR:C0J1Q3||Priesko4, Team ttKtt||2013-04-10 12:55:48 CDT||GO:0045916 negative regulation of complement activation (P)||PMID:22911815||ECO:0000314 direct assay evidence used in manual assertion|
Figure 3 shows the inhibition of both the alternative and classical pathways of complement activation through the inhibition of complement-mediated hemolysis of rabbit erthrocytes.
|ARTAU:Q6SJY7||Priesko4, Team ttKtt||2013-04-10 12:31:41 CDT||GO:0004040 amidase activity (F)||PMID:16885454||ECO:0000314 direct assay evidence used in manual assertion|
Figure 5 demonstrates the activity of Zn(II) and Co(II) trzN with selected s-triazines as substrates.
Figure 4 shows the effect of metal addition on ametryn hydrolysis activity of native TrzN purified from recombinant cells grown on 500M zinc and metal-depleted TrzN.
|HALMA:Q5V676||Kulhan11, Team ttKtt||2013-04-09 20:09:13 CDT||GO:0008106 alcohol dehydrogenase (NADP+) activity (F)||PMID:22015539||ECO:0000314 direct assay evidence used in manual assertion|
Table 2 depicts the HmADH12 gene followed Michaelis–Menten kinetics with the acetaldehyde substrate and NADP+ and NADPH cofactors in the reductive reaction. The enzyme was positively co-operative with ethanol as a substrate in the oxidative reaction.
|SALTY:Q9XDN0||Priesko4, Team ttKtt||2013-04-09 12:36:43 CDT||GO:0004022 alcohol dehydrogenase (NAD) activity (F)||PMID:23077559||ECO:0000315 mutant phenotype evidence used in manual assertion|
Figure 3 demonstrates the ability for the mutant to grow on media with minimal or saturated B12.
Figure 6 shows that PduQ regenerates NAD+ for the PduP reaction in purified Pdu MCPs by demonstrating the concentration of propionyl-CoA in comparison with a mutant phenotype.
|RHILE:Q20DQ4||Priesko4, Team ttKtt||2013-04-09 12:24:21 CDT||GO:0008378 galactosyltransferase activity (F)||PMID:22110131||ECO:0000314 direct assay evidence used in manual assertion|
Figure 3 demonstrates the rgtA activity through its ability to be a galA donor and viewing the total lipid extracts from strains with the enzyme vs. strains without the enzyme
|THESS:E8PQ06||Kulhan11, Team ttKtt||2013-04-09 12:13:05 CDT||GO:0016491 oxidoreductase activity (F)||PMID:22094050||ECO:0000314 direct assay evidence used in manual assertion|
Figure 5 depicts the ability of the ABC transporter, peptide-binding protein to reduce U(VI) in T. scotoductus SA-01. The reduction levels in the mutants with the protein was plotted against the reduction levels in the protein-free controls.
|PIRSE:Q9P8C9||Kulhan11, Team ttKtt||2013-04-09 11:06:19 CDT||GO:0044577 xylose catabolic process to ethanol (P)||PMID:22685138||ECO:0000315 mutant phenotype evidence used in manual assertion|
Figure 3 depicts oxygen-limited fermentation tests using the xylA mutants. Ethanol production was only present in the second- and third- round mutants, and xylose consumption was increased in these strains.
|PIRSE:Q9P8C9||Kulhan11, Team ttKtt||2013-03-28 18:22:10 CDT||GO:0009045 xylose isomerase activity (F)||PMID:22685138||ECO:0000315 mutant phenotype evidence used in manual assertion|
Table 2 depicts the wild-type xylose isomerase gene from Piromyces sp. and the first-, second-, and third-round mutants expressed in S. cerevisiae. In vitro xylose isomerase activity was measured and reported.
|HAEI8:MURA||Priesko4, Team ttKtt||2013-03-26 11:18:57 CDT||GO:0008760 UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity (F)||PMID:23462005||ECO:0000315 mutant phenotype evidence used in manual assertion|
Figure 3 demonstrates the inhibitory activities on MurA and then determines the residual activity.
Figure 5 demonstrates the inhibitory actions of sulfhydryl group dependent activity on the mutant phenotype.