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User:Kulhan11

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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptablePIRSE:Q9P8C92013-03-28 18:22:10 CDTGO:0009045 xylose isomerase activity (F)PMID:22685138ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2 depicts the wild-type xylose isomerase gene from Piromyces sp. and the first-, second-, and third-round mutants expressed in S. cerevisiae. In vitro xylose isomerase activity was measured and reported.

challenge
acceptablePIRSE:Q9P8C92013-04-09 11:06:19 CDTGO:0044577 xylose catabolic process to ethanol (P)PMID:22685138ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 depicts oxygen-limited fermentation tests using the xylA mutants. Ethanol production was only present in the second- and third- round mutants, and xylose consumption was increased in these strains.

challenge
updatedbyinstructorTHESS:E8PQ062013-04-09 12:13:05 CDTGO:0016491 oxidoreductase activity (F)PMID:22094050ECO:0000314 direct assay evidence used in manual assertion

Figure 5 depicts the ability of the ABC transporter, peptide-binding protein to reduce U(VI) in T. scotoductus SA-01. The reduction levels in the mutants with the protein was plotted against the reduction levels in the protein-free controls.

challenge
acceptableHALMA:Q5V6762013-04-09 20:09:13 CDTGO:0008106 alcohol dehydrogenase (NADP+) activity (F)PMID:22015539ECO:0000314 direct assay evidence used in manual assertion

Table 2 depicts the HmADH12 gene followed Michaelis–Menten kinetics with the acetaldehyde substrate and NADP+ and NADPH cofactors in the reductive reaction. The enzyme was positively co-operative with ethanol as a substrate in the oxidative reaction.

challenge
acceptableMETFE:G3P2013-04-12 19:05:05 CDTGO:0047100 glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity (F)PMID:10491162ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2 depicts kinetic parameters of both the wild-type and mutant M. fervidus GraP-DHs.

challenge
acceptableSERPL:Q8KKF52013-04-12 19:35:02 CDTGO:0008843 endochitinase activity (F)PMID:11734885ECO:0000314 direct assay evidence used in manual assertion

Figure 3 depicts the optimum temperature and pH for endochitinase activity.

challenge

acceptable:5
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Kulhan11

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