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PMID:22094050
| Citation |
Cason, ED, Piater, LA and van Heerden, E (2012) Reduction of U(VI) by the deep subsurface bacterium, Thermus scotoductus SA-01, and the involvement of the ABC transporter protein. Chemosphere 86:572-7 |
|---|---|
| Abstract |
In this study we investigated the effect of uranium on the growth of the bacterium Thermus scotoductus strain SA-01 as well as the whole cell U(VI) reduction capabilities of the organism. Also, site-directed mutagenesis confirmed the identity of a protein capable of a possible alternative mechanism of U(VI) reduction. SA-01 can grow aerobically in up to 1.25 mM uranium and has the capability to reduce low levels of U(VI) in under 20 h. TEM analysis performed on cells exposed to uranium showed extracellular and membrane-bound accumulation of uranium. The reductase-like protein was surprisingly identified as a peptide ABC transporter, peptide-binding protein. This study showcases the concept of protein promiscuity, where this protein with a distinct function in situ can also have the unintended function of a reactant for the reduction of U(VI). |
| Links |
PubMed Online version:10.1016/j.chemosphere.2011.10.006 |
| Keywords |
ATP-Binding Cassette Transporters/chemistry; ATP-Binding Cassette Transporters/genetics; ATP-Binding Cassette Transporters/metabolism; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Biodegradation, Environmental; Electron Transport; Environmental Pollutants/isolation & purification; Environmental Pollutants/metabolism; Environmental Pollutants/toxicity; Models, Molecular; Mutation; Oxidoreductases/isolation & purification; Oxidoreductases/metabolism; Protein Conformation; Thermus/cytology; Thermus/drug effects; Thermus/enzymology; Thermus/metabolism; Uranium/isolation & purification; Uranium/metabolism; Uranium/toxicity |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0016491: oxidoreductase activity |
ECO:0000314: |
F |
Figure 5 depicts the ability of the ABC transporter, peptide-binding protein to reduce U(VI) in T. scotoductus SA-01. The reduction levels in the mutants with the protein was plotted against the reduction levels in the protein-free controls. |
complete | ||||
|
enables |
GO:0016491: oxidoreductase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
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