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User:Mill2034

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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorARATH:DSP42013-03-28 12:19:44 CDTGO:0005982 starch metabolic process (P)PMID:19141707ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1 shows SEX4 incubated with different amounts of prephosphorylated starch granules caused dephosphorylates the starch granule surface. Activity increased with starch added. Activity decreased when leaf extracts from mutants sex4 when compared to wild type extracts.

challenge
acceptableECO57:HHA2013-04-09 20:56:09 CDTGO:1900191 negative regulation of single-species biofilm formation (P)PMID:23377937ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 shows when you have a hha mutant, biofilm formation increases but when you complement the mutant with hha+, biofilm formation goes down, as hha is a negative regulator of biofilm production

challenge
updatedbyinstructorECOLI:CPXR2013-04-09 21:30:27 CDTGO:0045893 positive regulation of transcription, DNA-dependent (P)PMID:15743952ECO:0000314 direct assay evidence used in manual assertion

Fig 5.

challenge
updatedbyinstructorARATH:AB1D2013-04-10 16:42:22 CDTGO:0016042 lipid catabolic process (P)PMID:12065405ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 shows failure of lipid breakdown in germinated cotyledons in cts-1 mutants. Figure 5 shows TAG-derived fatty acids and acyl CoA levels in wild type and cts-1 and cts-2 mutants. There was much less breakdown of fatty acids in the mutants

challenge
acceptableARATH:MSI52013-04-13 13:53:29 CDTGO:0010629 negative regulation of gene expression (P)PMID:22102827ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 and 3 both show how the gene negatively regulates the expression of other genes by looking at the expression of those genes in wild type and mutant plants.

challenge
requireschanges?2013-04-13 14:02:27 CDTGO:0010629 negative regulation of gene expression (Figure 8 shows that when a mutant for the gene is made, there is an increase in expression of the gene regulated by HDA6)PMID:22102827IMP: Inferred from Mutant Phenotype
challenge
unacceptableHUMAN:Q6NVY42013-04-14 14:27:45 CDTGO:0000975 regulatory region DNA binding (F)PMID:23579274ECO:0000314 direct assay evidence used in manual assertion

Figure 3 shows how the protein binds to DNA at a specific region in the DR5 promoter, through oligo binding and Western blots

challenge
updatedbyinstructorARATH:TCP182013-04-14 14:45:16 CDTGO:0010223 secondary shoot formation (P)PMID:23524661ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 1 & 3.

challenge

acceptable:2
unacceptable:1
requires_changes:1
flagged:0

What do the icons mean in the status column?

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