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User:Brcalk

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CACAO Spring 2015

  • 4 Annotations
  • 17 Challenges
  • 0 Credits through NTRs
  • 21 Annotations total


My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
correctedthroughchallengesCYPCA:Q98SI32015-02-03 20:27:54 CSTGO:0051873 killing by host of symbiont cells (P)PMID:25251775ECO:0000314 direct assay evidence used in manual assertion

As seen in figure 6, when E. coli culture is treated with Apo-A1, CFU numbers are affected in a comparable manner to the antibiotic oxytetracyclin.

challenge
acceptableCYPCA:Q98SI32015-03-24 19:45:16 CDTGO:0005737 cytoplasm (C)PMID:25251775ECO:0000314 direct assay evidence used in manual assertion

In the liver, strong positive immunostaining for ApoA-I was localized to the cytoplasm of hepatocytes (Fig. 5G).

challenge
unacceptableCYPCA:I1XUS22015-03-24 20:10:39 CDTGO:0005737 cytoplasm (C)PMID:25251775ECO:0000314 direct assay evidence used in manual assertion

As seen in figure 5H: "In the liver cells the positive strong signal for ApoA-I is localized to the hepatocyte cytoplasm (arrows). Weak positive staining for Apo-14 kDa is visible (arrow)."

challenge
acceptableTAKPA:SXT12015-03-24 21:07:17 CDTGO:0005737 cytoplasm (C)PMID:23769753ECO:0000314 direct assay evidence used in manual assertion

As seen in Figure 2, the protein was localized in the cytoplasm of the liver cells.

challenge
acceptableANAPL:D3TI842015-03-27 16:21:52 CDTGO:0005737 cytoplasm (C)PMID:23916689ECO:0000314 direct assay evidence used in manual assertion

The duRIG-I protein was evenly distributed in cells (Figure 1 A), especially in the cytoplasm and nucleus.

challenge
unacceptableDANRE:Q9W7672015-03-31 20:18:51 CDTGO:0005829 cytosol (C)PMID:25803551ECO:0000314 direct assay evidence used in manual assertion

" These experiments show co-localization of the GFP and Mdka immunofluorescence, demonstrating that in horizontal cells the Mdka immunolabel is cytosolic (Fig. 2A). "

challenge
unacceptableDANRE:Q9W7672015-03-31 20:22:42 CDTGO:0005737 cytoplasm (C)PMID:25803551ECO:0000314 direct assay evidence used in manual assertion

"As seen in Figure 2, immunolabels for both Mdka and EGFP colocalize to the cytoplasm of horizontal cells (arrowhead, panel A). "

challenge
acceptableDANRE:Q9W7672015-03-31 20:22:42 CDTGO:0005794 Golgi apparatus (C)PMID:25803551ECO:0000314 direct assay evidence used in manual assertion

As seen in Figure 2, "In the adult retina, Mdka immunostaining colocalizes with the antibody marker of the Golgi complex (column B; and insets)"

challenge
correctedthroughchallengesMOUSE:PAR22015-03-31 21:12:37 CDTGO:0005634 nucleus (C)PMID:25216639ECO:0000314 direct assay evidence used in manual assertion

Figure 2A

challenge
correctedthroughchallengesMOUSE:PAR22015-03-31 21:12:37 CDTGO:0005886 plasma membrane (C)PMID:25216639ECO:0000314 direct assay evidence used in manual assertion

Figure 2A

challenge

acceptable:4
unacceptable:3
requires_changes:0
flagged:0

Annotations challenged by Brcalk

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
unacceptableJderrickson,
Team Bio Bois		THAPS:B8BWV6GO:0033681 - ATP-dependent 3'-5' DNA/RNA helicase activity (F)25579795:25579795ECO:0000314 direct assay evidence used in manual assertionchallengeC: 5
unacceptableBwagner,
Team Plants Just Wanna Have Sun		ECOLI:MUTSGO:0008301 - DNA binding, bending (F)PMID:24101514ECO:0000317 genomic context evidence used in manual assertionchallengeC: 2
unacceptableAsingh,
Team Designated Drinkers		LACCA:K0N2B5GO:0051117 - ATPase binding (F)PMID:25658360ECO:0000314 direct assay evidence used in manual assertionchallengeC: 3
correctedthroughchallengesAsingh,
Team Designated Drinkers		HUMAN:TNNC1GO:0030172 - troponin C binding (F)PMID:7852318ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 6

fixed by Brcalk
unacceptableAsingh,
Team Designated Drinkers		HUMAN:TNNT2GO:0031014 - troponin T binding (F)PMID:25646632ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2
correctedthroughchallengesMrogers,
Team Dos Juanitas		HUMAN:NBR1GO:0051019 - mitogen-activated protein kinase binding (F)PMID:25659039ECO:0000314 direct assay evidence used in manual assertionchallengeC: 3

fixed by Brcalk
correctedthroughchallengesKsenagbe,
Team Mic Drop		SALTI:HLYEGO:0016021 - integral component of membrane (C)PMID:18439098ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 7

fixed by Brcalk
correctedthroughchallengesBrcalk,
Team Swanson		CYPCA:Q98SI3GO:0051873 - killing by host of symbiont cells (P)PMID:25251775ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2

fixed by Brcalk
correctedthroughchallengesBbourgoyne,
Team Bio Bois		BRADU:NIFHGO:0016163 - nitrogenase activity (F)PMID:24062462ECO:0000314 direct assay evidence used in manual assertionchallengeC: 10

fixed by JChamblee1995
correctedthroughchallengesJderrickson,
Team Bio Bois		HUMAN:MASP1GO:0008236 - serine-type peptidase activity (F)PMID:25617734ECO:0000314 direct assay evidence used in manual assertionchallengeC: 6

fixed by Brcalk
correctedthroughchallengesJderrickson,
Team Bio Bois		HUMAN:MGAGO:0004558 - alpha-1,4-glucosidase activity (F)PMID:25326060ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2

fixed by Brcalk
correctedthroughchallengesBbourgoyne,
Team Bio Bois		ENTAE:AMOGO:0008131 - primary methylamine oxidase activity (F)PMID:24672387ECO:0000314 direct assay evidence used in manual assertionchallengeC: 5

fixed by Brcalk
requireschangesLperilloux,
Team Ubiquitous Mafia		HUMAN:MTORGO:1902949 - positive regulation of tau-protein kinase activity (P)PMID:23585566ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
correctedthroughchallengesLperilloux,
Team Ubiquitous Mafia		DROME:Q7KTX7GO:0031398 - positive regulation of protein ubiquitination (P)PMID:20194754ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1

fixed by Brcalk
unacceptableSedmondson,
Team Designated Drinkers		SALTI:RNTGO:0004518 - nuclease activity (F)PMID:22735700ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2
requireschangesSedmondson,
Team Designated Drinkers		HUMAN:CUL5GO:0016567 - protein ubiquitination (P)PMID:23304509ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
correctedthroughchallengesKsenagbe,
Team Mic Drop		ECOLI:ARCBGO:0016021 - integral component of membrane (C)PMID:15326287ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1

fixed by Brcalk
correctedthroughchallengesKsenagbe,
Team Mic Drop		HUMAN:ABCE1GO:0008428 - ribonuclease inhibitor activity (F)PMID:25659154ECO:0000314 direct assay evidence used in manual assertionchallengeC: 3

fixed by Brcalk
correctedthroughchallengesMin.lorna,
Team Ood		MOUSE:RHOAGO:0045820 - negative regulation of glycolytic process (P)PMID:25398325ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1

fixed by Brcalk

11 annotations fixed by Brcalk

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