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PMID:15326287

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Citation

Malpica, R, Franco, B, Rodriguez, C, Kwon, O and Georgellis, D (2004) Identification of a quinone-sensitive redox switch in the ArcB sensor kinase. Proc. Natl. Acad. Sci. U.S.A. 101:13318-23

Abstract

Escherichia coli senses and signals anoxic or low redox conditions in its growth environment by the Arc two-component system. Under anaerobic conditions, the ArcB sensor kinase autophosphorylates and transphosphorylates ArcA, a global transcriptional regulator that controls the expression of numerous operons involved in respiratory or fermentative metabolism. Under aerobic conditions, the kinase activity of ArcB is inhibited by the quinone electron carriers that act as direct negative signals. Here, we show that the molecular mechanism of kinase silencing involves the oxidation of two cytosol-located redox-active cysteine residues that participate in intermolecular disulfide bond formation, a reaction in which the quinones provide the source of oxidative power. Thus, a pivotal link in the Arc signal transduction pathway connecting the redox state of the quinone pool to the transcriptional apparatus is elucidated.

Links

PubMed PMC516565 Online version:10.1073/pnas.0403064101

Keywords

Dimerization; Disulfides/chemistry; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Membrane Proteins/chemistry; Membrane Proteins/metabolism; Oxidation-Reduction; Protein Kinases/chemistry; Protein Kinases/metabolism; Quinones/pharmacology; Signal Transduction

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:ARCB

acts_upstream_of_or_within

GO:0070482: response to oxygen levels

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:ARCB

GO:0016021: integral component of membrane

ECO:0000314:

C

Figure 1: ArcB possesses a cytoplasm-located signal reception site, it is activated only by the membrane-permeating thiol-reductants during aerobiosis, and has two unique cysteine residues in the linker region

complete
CACAO 10733

Notes

See also

References

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