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PMID:25659154

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Citation

Kärblane, K, Gerassimenko, J, Nigul, L, Piirsoo, A, Smialowska, A, Vinkel, K, Kylsten, P, Ekwall, K, Swoboda, P, Truve, E and Sarmiento, C (2015) ABCE1 Is a Highly Conserved RNA Silencing Suppressor. PLoS ONE 10:e0116702

Abstract

ATP-binding cassette sub-family E member 1 (ABCE1) is a highly conserved protein among eukaryotes and archaea. Recent studies have identified ABCE1 as a ribosome-recycling factor important for translation termination in mammalian cells, yeast and also archaea. Here we report another conserved function of ABCE1. We have previously described AtRLI2, the homolog of ABCE1 in the plant Arabidopsis thaliana, as an endogenous suppressor of RNA silencing. In this study we show that this function is conserved: human ABCE1 is able to suppress RNA silencing in Nicotiana benthamiana plants, in mammalian HEK293 cells and in the worm Caenorhabditis elegans. Using co-immunoprecipitation and mass spectrometry, we found a number of potential ABCE1-interacting proteins that might support its function as an endogenous suppressor of RNA interference. The interactor candidates are associated with epigenetic regulation, transcription, RNA processing and mRNA surveillance. In addition, one of the identified proteins is translin, which together with its binding partner TRAX supports RNA interference.

Links

PubMed Online version:10.1371/journal.pone.0116702

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:ERI1

involved_in

GO:1900369: negative regulation of post-transcriptional gene silencing by RNA

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:ABCE1

GO:0008428: ribonuclease inhibitor activity

ECO:0000314:

F

Fig 1. ABCE1 suppresses RNAi of GFP in N. benthamiana. Fig 2. Northern blot analysis showing the suppression of GFP RNA silencing in N. benthamiana by ABCE1

complete
CACAO 10631

Notes

See also

References

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