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Category:Team Mic Drop

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesECOLI:NRFAKsenagbe, Team Mic Drop2015-03-10 16:45:50 CDTGO:0003028 regulation of systemic arterial blood pressure by aortic body chemoreceptor signaling (P)PMID:18375814ECO:0000314 direct assay evidence used in manual assertion

Figure 1&2: activity assays and measurements of NO consumption showing that the increased nitrite reductase activity correlates with an elevated capacity for NO reduction by intact cells.

challenge
unacceptableDICDI:ABCD2Ksenagbe, Team Mic Drop2015-03-10 16:38:37 CDTGO:0006955 immune response (P)PMID:20083612ECO:0000314 direct assay evidence used in manual assertion

Figure 4: Diagram of the traJ-traY border in the pSLT plasmid, indicating the position of ptraY, the region homologous to ArcA binding sites of other F-like plasmids, and the locations of the primers used for PCR amplification.

challenge
correctedthroughchallengesECOLI:ARCBKsenagbe, Team Mic Drop2015-03-10 16:26:08 CDTGO:0016021 integral component of membrane (C)PMID:15326287ECO:0000314 direct assay evidence used in manual assertion

Figure 1: ArcB possesses a cytoplasm-located signal reception site, it is activated only by the membrane-permeating thiol-reductants during aerobiosis, and has two unique cysteine residues in the linker region

challenge
requireschangesSALTM:P97126Ksenagbe, Team Mic Drop2015-03-10 13:03:28 CDTGO:0071346 cellular response to interferon-gamma (P)PMID:17526833ECO:0000314 direct assay evidence used in manual assertion

Figure 1: rnr transcript and Hmp protein levels in Salmonella mutants

challenge
requireschangesECOLI:NORRKsenagbe, Team Mic Drop2015-03-10 10:45:59 CDTGO:0050421 nitrite reductase (NO-forming) activity (F)PMID:18003617ECO:0000316 genetic interaction evidence used in manual assertion

FIGURE 2: Transcriptional activation in vivo by NorR and mutant derivatives and stability of mutant proteins.

challenge
requireschangesBACIU:J7JTG7Ksenagbe, Team Mic Drop2015-03-10 10:29:09 CDTGO:0046679 response to streptomycin (Figure 1: The apo- and [Fe-S]-NsrR interact with two distinct classes of DNA targets that have different recognition sequences.)PMID:21091510IDA: Inferred from Direct Assay
challenge
requireschangesECOHS:NSRRKsenagbe, Team Mic Drop2015-03-10 10:18:36 CDTGO:1900488 negative regulation of [2Fe-2S] cluster assembly (P)PMID:8989365ECO:0000314 direct assay evidence used in manual assertion

Figure 3 : DNA binding assays with the hmpA1 and hmpA2 promoters and purified NsrR protein.shows the loss of DNA binding activity. This is the first demonstration that NsrR contains an NO-sensitive [2Fe-2S] cluster that is required for DNA binding activity.

challenge
unacceptableECOLI:FNRKsenagbe, Team Mic Drop2015-03-10 02:30:50 CDTGO:0005737 cytoplasm (C)PMID:22029434ECO:0000314 direct assay evidence used in manual assertion

Fig 1: The FNR protein regulates transitions between aerobic and anaerobic growth and also a physiologically relevant sensor of NO

challenge
unacceptableOCHAN:C0A1E1Ksenagbe, Team Mic Drop2015-03-10 02:17:32 CDTGO:1900079 regulation of arginine biosynthetic process (P)PMID:16045618ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1: with NssR being a Crp-Fnr superfamily member, an Fnr-like binding sequence (TTAAC-N(4)-GTTAA) was found upstream of each gene at locations -40.5 to -42.5 relative to the centre of the binding sites and the transcription start point.

challenge
requireschangesECOLI:NSRRKsenagbe, Team Mic Drop2015-03-10 02:07:10 CDTGO:0005737 cytoplasm (C)PMID:19656291ECO:0000314 direct assay evidence used in manual assertion

Fig 2: NsrR binds to sites in the promoter regions of the fliAZY, fliLMNOPQR and mqsR-ygiT transcription units, which encode proteins involved in motility and biofilm development.

challenge
correctedthroughchallengesSALTI:HLYEKsenagbe, Team Mic Drop2015-03-10 01:57:17 CDTGO:0016021 integral component of membrane (C)PMID:18439098ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 1: the SARB collection shown to cause systemic infections in humans have SPI-18 and hlyE and express an active hemolysin revealed upon bacterial envelope destabilization.

challenge
unacceptableECO57:HLYEKsenagbe, Team Mic Drop2015-03-10 01:46:18 CDTGO:0019836 hemolysis by symbiont of host erythrocytes (P)PMID:14996792ECO:0000314 direct assay evidence used in manual assertion

Fig 2: FNR and CRP make positive contributions, whereas H-NS acts negatively under these conditions.

challenge
unacceptableARATH:DDLKsenagbe, Team Mic Drop2015-03-10 01:31:24 CDTGO:0005515 protein binding (F)PMID:25738598ECO:0000314 direct assay evidence used in manual assertion

Figure 1: the binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate. B50% represents the concentration of Ab required to generate 50% of maximum binding.

challenge
unacceptableHUMAN:DUX4CKsenagbe, Team Mic Drop2015-03-10 00:49:11 CDTGO:0001404 invasive growth (P)PMID:25743594ECO:0000314 direct assay evidence used in manual assertion

Figure 3: The tumour tissues and cancer cell lines revealed that Nup214 and miR-133b expressions are negatively correlated. In vitro, Nup214 was significantly downregulated by ectopic miR-133b.

challenge
requireschangesHUMAN:KCNE2Ksenagbe, Team Mic Drop2015-02-24 17:53:32 CSTGO:0006811 ion transport (P)PMID:22880098ECO:0000247 sequence alignment evidence used in manual assertion

Figure 4: Activation time constants for Ih in pyramidal cells were fast in both genotypes compared to those in VB neurons, Kcne2 deletion again increased time constants in pyramidal neurons

Figure 6: he frequency of spontaneous EPSPs in layer 6 pyramidal neurons in the absence of 4-AP was increased by Kcne2 deletion, suggesting a possible alteration in glutamate release machinery.

challenge
unacceptableRAT:HCN2Ksenagbe, Team Mic Drop2015-02-24 17:41:34 CSTGO:0006810 transport (P)PMID:22948144ECO:0000314 direct assay evidence used in manual assertion

Figure 4: agarose gel for HCN1 PCR indicating amplification from the cochlear inner and outer hair cell cDNA of predicted 397-bp product crossing intron (white arrows).

challenge
requireschangesHUMAN:CAMPKsenagbe, Team Mic Drop2015-02-24 12:48:53 CSTGO:0001934 positive regulation of protein phosphorylation (P)PMID:24085296ECO:0000266 sequence orthology evidence used in manual assertion

Figure 1: Fisetin potentiation of HCN2 channels resembled the channel's regulation by cAMP, thereby suggesting that fisetin and cAMP may use similar mechanisms to stabilize the channel's open state

challenge
requireschangesHAEIF:LIC2BKsenagbe, Team Mic Drop2015-02-23 23:52:13 CSTGO:0009103 lipopolysaccharide biosynthetic process (P)PMID:21357723ECO:0000255 match to sequence model evidence used in manual assertion

Figure 3: with two independently derived clones for each mutation, lic2B and lic2C mutants yielded marked and statistically significant decreases in survival compared to the wild type

challenge
unacceptableECOLI:HLYEKsenagbe, Team Mic Drop2015-02-23 23:28:51 CSTGO:0009405 pathogenesis (P)PMID:10858262ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1: Cytotoxicity as manifested by LDH release from murine macrophages J774.

Figure 2: The viability of freshly isolated human peripheral monocytes showed a dose-related decline after treatment with ClyA for 20 h

challenge
requireschangesECOLI:FERKsenagbe, Team Mic Drop2015-02-23 23:05:12 CSTGO:1900489 positive regulation of [2Fe-2S] cluster assembly (P)PMID:23019358ECO:0000247 sequence alignment evidence used in manual assertion

Fig. 2.

Resonance Raman spectra of the O2-induced [4Fe-4S]2+ to [2Fe-2S]2+ cluster conversion of FNR with natural abundance (black spectra) and 34S-labeled (red spectra) bridging sulfides. (A) [4Fe-4S]-FNR prepared by anaerobic reconstitution.

challenge
requireschangesECOLW:E0IUS3Ksenagbe, Team Mic Drop2015-02-23 22:49:57 CSTGO:0005737 cytoplasm (C)PMID:16339951ECO:0000314 direct assay evidence used in manual assertion

Fig. 1. Activation of an FNR-dependent model promoter upon transfer to anaerobic conditions in the absence of de novo FNR synthesis. The expression of fnr in JRG5438 was controlled by the thermo-inducible (42 °C) promoter of pGS199, and FNR activity was reported by activation of the FNR-dependent FF-41.5 promoter fused to the reporter gene lacZ.

challenge
requireschangesMOUSE:GIT1Ksenagbe, Team Mic Drop2015-02-22 01:26:47 CSTGO:0005096 GTPase activator activity (F)PMID:25694429ECO:0000314 direct assay evidence used in manual assertion

αPIX overexpression in the absence of IL-2 resulted in a significant increase of the PYGM activity when compared to control cells (Figure 1A, second empty bar compared to first empty bar). Western blot shows αPIX expression levels

As shown in Figure 2 A, in the absence of αPIX (knockdown), IL-2 was unable to stimulate Rac 1 activation

challenge
unacceptableMOUSE:KLOTKsenagbe, Team Mic Drop2015-02-22 00:57:15 CSTGO:0005104 fibroblast growth factor receptor binding (F)PMID:25695625ECO:0000314 direct assay evidence used in manual assertion

Figure 1: Klotho expression was suppressed in the diabetic rats, but was increased by rAAV.mKL delivery. rAAV.mKL significantly suppressed diabetes‑induced renal hypertrophy and histopathological changes, reduced renal collagen fiber generation and decreased kidney hypertrophy index.

challenge
requireschangesHUMAN:MDM4Ksenagbe, Team Mic Drop2015-02-20 00:17:40 CSTGO:0000122 negative regulation of transcription from RNA polymerase II promoter (P)PMID:25659040ECO:0000314 direct assay evidence used in manual assertion

Figure 1. The hMDMXp60 isoform is initiated at the 7th in frame AUG codon. (A) Cartoon illustrating the hmdmx mRNA constructs and the mutated AUG sites

Figure 2. hMDMXp60 and hMDMXFL are derived from cap-independent mRNA translation initiation. Figure 3. hMDMXp60 shows higher affinity toward hMDM2 as compared to hMDMXFL. Immunoprecipitation (IP) against hMDM2 shows that hMDM2 interacts with both hMDMX isoforms

challenge
unacceptableHUMAN:DGCR8Ksenagbe, Team Mic Drop2015-02-19 23:55:34 CSTGO:0005737 cytoplasm (C)PMID:25670082ECO:0000314 direct assay evidence used in manual assertion

Figure 1. Comprehensive analysis of 77 FHWTs indicates that tumors with SIX1/2 and/or miRNAPG mutations show a pre-induction metanephric mesenchyme gene expression pattern and are significantly associated with both perilobar nephrogenic rests and 11p15 imprinting aberrations.

Figure 2. The combination of SIX and miRNAPG mutations in the same tumor is associated with evidence of RAS activation and a higher rate of relapse and death.

challenge
requireschangesECOLI:DOSCMharrelson, Team Mic Drop2015-02-10 17:04:20 CSTGO:0042710 biofilm formation (P)PMID:25012525ECO:0000314 direct assay evidence used in manual assertion

Figure 1: Characterization of Biofilm Formation

challenge
requireschangesECOD1:C9QT00Mharrelson, Team Mic Drop2015-02-10 17:02:17 CSTGO:0042710 biofilm formation (Figure 1: Characterization of Biofilm Producers)PMID:25012525IDA: Inferred from Direct Assay
challenge
requireschangesECO57:Q8X5C2Mharrelson, Team Mic Drop2015-02-10 17:00:29 CSTGO:0042710 biofilm formation (P)PMID:25012525ECO:0000314 direct assay evidence used in manual assertion

Figure 1: Characterization of Biofilm Producers

challenge
requireschangesECO27:B7UQV9Mharrelson, Team Mic Drop2015-02-10 16:58:24 CSTGO:0042710 biofilm formation (P)PMID:25012525ECO:0000314 direct assay evidence used in manual assertion

Figure 1: Characterization of Biofilm Producers

challenge
correctedthroughchallengesHUMAN:ABCE1Ksenagbe, Team Mic Drop2015-02-10 12:49:53 CSTGO:0008428 ribonuclease inhibitor activity (F)PMID:25659154ECO:0000314 direct assay evidence used in manual assertion

Fig 1. ABCE1 suppresses RNAi of GFP in N. benthamiana.

Fig 2. Northern blot analysis showing the suppression of GFP RNA silencing in N. benthamiana by ABCE1

challenge
requireschangesHUMAN:RANB9Ksenagbe, Team Mic Drop2015-02-09 21:44:14 CSTGO:0005829 cytosol (C)PMID:25659156ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 1. Deletion of RanBPM C-terminus does not alter its sub cellular localization.

Fig 2. RanBPM N-terminal region contains several determinants that regulate its subcellular localization.

challenge

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Pages in category "Team Mic Drop"

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