PMID:23916689

Citation	Chen, Y, Zhang, Y, Huang, Z, Xu, Q, Zhu, Z, Tong, Y, Yu, Q, Ding, J and Chen, G (2013) Molecular characterization, expression patterns, and subcellular localization of RIG-I in the Jinding duck (Anas platyrhynchos domesticus). Dev. Comp. Immunol. 41:766-71
Abstract	Retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) have recently been identified as cytoplasmic sensors for RNA virus. Recent research has shown that RIG-I, a member of this family, play an important role in innate immunity. In this study, we cloned the RIG-I gene from Jinding duck by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). We determined that the cDNA of duRIG-I contains a 14-bp 5' UTR, a 2802-bp open reading frame, and alternative 3' UTRs (295-bp and 927-bp) and encodes a polypeptide of 933 amino acids. Based on this sequence, the duRIG-I protein is predicted to have conserved domains typical of RLRs. In addition, duRIG-I was found to be distributed throughout DF1 cells by indirect immunofluorescence, as predicted. duRIG-I mRNA was scarcely detected in healthy tissues by semi-quantitative RT-PCR (sqRT-PCR). To study the role of RIG-I in innate immunity, we used synthetic double-stranded RNA to mimic viral infection in vivo and detected duRIG-I transcripts in spleen and liver by quantitative real-time PCR (qRT-PCR). The expression of duRIG-I mRNA was significantly elevated at 8h post-injection (P < 0.05) and was indistinguishable from control levels at other time points (P > 0.05). These results suggest that duRIG-I plays an important role in innate immune responses to double-stranded RNA viruses and warrant further studies to reveal the possible mechanism.
Links	PubMed Online version:10.1016/j.dci.2013.07.018
Keywords	Amino Acid Sequence; Animals; Cell Line; Cloning, Molecular/methods; DEAD-box RNA Helicases/biosynthesis; DEAD-box RNA Helicases/genetics; DEAD-box RNA Helicases/immunology; DEAD-box RNA Helicases/metabolism; DNA, Complementary/genetics; Ducks/genetics; Ducks/immunology; Ducks/metabolism; Immunity, Innate/genetics; Immunity, Innate/immunology; Liver/immunology; Liver/metabolism; Phylogeny; RNA, Double-Stranded/genetics; RNA, Double-Stranded/immunology; RNA, Messenger/genetics; Sequence Alignment; Sequence Homology, Amino Acid; Spleen/immunology; Spleen/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ANAPL:D3TI84		GO:0005737: cytoplasm	ECO:0000314:		C		The duRIG-I protein was evenly distributed in cells (Figure 1 A), especially in the cytoplasm and nucleus.	complete CACAO 10798
ANAPL:D3TI84	part_of	GO:0005737: cytoplasm	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete

References

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