PMID:25251775

Citation	Dietrich, MA, Adamek, M, Bilińska, B, Hejmej, A, Steinhagen, D and Ciereszko, A (2014) Characterization, expression and antibacterial properties of apolipoproteins A from carp (Cyprinus carpio L.) seminal plasma. Fish Shellfish Immunol. 41:389-401
Abstract	Apolipoproteins A are multifunctional proteins that, in addition to contributing to lipid metabolism and transport, are associated with the innate immune system in fish. Using a three step isolation procedure consisting of affinity chromatography on Blue-Sepharose, delipidation and reverse phase HPLC we isolated apolipoproteins from carp seminal plasma and identified them as ApoA-I and Apo-14 kDa. Moreover, we provided the full-length cDNA sequence of ApoA-I encoding 257 amino acids including a 18 amino acid signal peptide and a 4 amino acid propeptide. Apolipoproteins corresponded to the most abundant proteins in carp seminal plasma. Both ApoA-I and Apo-14 kDa were represented by several proteoforms that differ both in molecular mass and isoelectric point. The proteoforms of ApoA-I characteristic for seminal plasma were distinguished from those of blood. Carp seminal plasma ApoA-I and Apo-14 kDa showed a high immunologic similarity to their counterparts in carp blood and seminal plasma of other Cyprinid species. The mRNA expression analysis and immunohistochemical study suggest synthesis and secretion of ApoA-I and Apo-14 kDa in the fish reproductive tract and suggest a role in spermatogenesis and the stabilization of sperm membrane. Moreover, ApoA-I displayed bactericidal activity against Escherichia coli and bacteriostatic activity against Aeromonas hydrophila which suggests that ApoA-I is associated with innate immune system of the fish reproductive tract.
Links	PubMed Online version:10.1016/j.fsi.2014.09.020
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
CYPCA:Q98SI3	Contributes to	GO:0051873: killing by host of symbiont cells	ECO:0000314:		P		As seen in figure 6, when E. coli culture is treated with Apo-A1, CFU numbers are affected in a comparable manner to the antibiotic oxytetracyclin.	complete CACAO 10543
CYPCA:Q98SI3		GO:0005737: cytoplasm	ECO:0000314:		C		In the liver, strong positive immunostaining for ApoA-I was localized to the cytoplasm of hepatocytes (Fig. 5G).	complete CACAO 10779
CYPCA:Q98SI3	part_of	GO:0005737: cytoplasm	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
CYPCA:I1XUS2		GO:0005737: cytoplasm	ECO:0000314:		C		As seen in figure 5H: "In the liver cells the positive strong signal for ApoA-I is localized to the hepatocyte cytoplasm (arrows). Weak positive staining for Apo-14 kDa is visible (arrow)."	complete CACAO 10780

References

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