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PMID:22735700

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Citation

Thorn, A, Steinfeld, R, Ziegenbein, M, Grapp, M, Hsiao, HH, Urlaub, H, Sheldrick, GM, Gärtner, J and Krätzner, R (2012) Structure and activity of the only human RNase T2. Nucleic Acids Res. 40:8733-42

Abstract

Mutations in the gene of human RNase T2 are associated with white matter disease of the human brain. Although brain abnormalities (bilateral temporal lobe cysts and multifocal white matter lesions) and clinical symptoms (psychomotor impairments, spasticity and epilepsy) are well characterized, the pathomechanism of RNase T2 deficiency remains unclear. RNase T2 is the only member of the Rh/T2/S family of acidic hydrolases in humans. In recent years, new functions such as tumor suppressing properties of RNase T2 have been reported that are independent of its catalytic activity. We determined the X-ray structure of human RNase T2 at 1.6 Å resolution. The α+β core fold shows high similarity to those of known T2 RNase structures from plants, while, in contrast, the external loop regions show distinct structural differences. The catalytic features of RNase T2 in presence of bivalent cations were analyzed and the structural consequences of known clinical mutations were investigated. Our data provide further insight into the function of human RNase T2 and may prove useful in understanding its mode of action independent of its enzymatic activity.

Links

PubMed PMC3458558 Online version:10.1093/nar/gks614

Keywords

Amino Acid Sequence; Binding Sites; Copper/pharmacology; Crystallography, X-Ray; Endoribonucleases/chemistry; Endoribonucleases/genetics; Endoribonucleases/metabolism; Glycosylation; Humans; Models, Molecular; Molecular Sequence Data; Mutation; Protein Folding; Structural Homology, Protein; Zinc/chemistry; Zinc/pharmacology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

SALTI:RNT

GO:0004518: nuclease activity

ECO:0000314:

F

This paper provides necessary information about the function and structure of Ribonuclease T2 and includes Figures 1-6 that are used to thoroughly display need evidence.

complete
CACAO 10685

HUMAN:RNT2

enables

GO:0004540: ribonuclease activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:RNT2

involved_in

GO:0006401: RNA catabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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