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User:Schmi409

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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableECOLI:PURU2013-03-29 01:56:31 CDTGO:0006164 purine nucleotide biosynthetic process (P)PMID:8226647ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2. Mutants of the purU gene, along with the wild-type strains of E.coli were grown on medium containing various amino acids. Mutants required purine or formate supplementation for growth indicating role of purU in de novo purine nucleotide biosynthesis.

challenge
updatedbyinstructorROSHC:Q5I6B52013-04-08 09:15:21 CDTGO:0004026 alcohol O-acetyltransferase activity (F)PMID:12692346ECO:0000314 direct assay evidence used in manual assertion

Figure 4: Shows that the RhAAT1 gene product catalyzed the production of geranyl acetate from gerniol and aceyl-CoA and that this was dependent on the presence of the RhAAT1 gene product (enzyme).

challenge
updatedbyinstructorMAIZE:BX62013-04-08 09:31:25 CDTGO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (F)PMID:12620350ECO:0000314 direct assay evidence used in manual assertion

Figure 2: Shows that the inhibition of 2-oxoglutarate-dependent dioxygenases significantly decreases the production of DIMBOA

challenge
acceptableBACSU:GLK2013-04-08 13:24:46 CDTGO:0004340 glucokinase activity (F)PMID:9620975ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2: Shows that a mutant strain MD186 (affecting the glcK gene) has complete loss of glucose kinase activity

challenge
updatedbyinstructorRHISY:A7BJ352013-04-09 19:24:44 CDTGO:0016871 cycloartenol synthase activity (F)PMID:17617700ECO:0000314 direct assay evidence used in manual assertion

Figure 2: Shows that when the RsCAS gene was introduced into yeast, cycloartenols were produced

challenge
requireschangesVANPL:Q6Q7962013-04-11 14:36:10 CDTGO:0047763 caffeate O-methyltransferase activity (F)PMID:15118832ECO:0000021 physical interaction evidence

Table 1: Shows that culture crude protein extracts demonstrated caffeic acid O-methyltransferase activity.

challenge
acceptable9SPHN:G2IMC62013-04-11 18:52:12 CDTGO:0050608 vanillin dehydrogenase activity (F)PMID:17928721ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4B: Shows that ligV-inactivation mutant (DLV) did not have significant growth on vanillin, yet the wild-type strain SYK-6 did have significant growth on vanillin

challenge
updatedbyinstructorPSEUH:VDH2013-04-14 11:07:57 CDTGO:0050608 vanillin dehydrogenase activity (F)PMID:9098058ECO:0000314 direct assay evidence used in manual assertion

Figure 10: Shows that the recombinant E.coli XL1-Blue strains with vdh gene converted vanillin to vanillic acid.

challenge

acceptable:3
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Schmi409

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
updatedbyinstructorOekshyyan,
Team Pancakes at Night
MOUSE:IGF2GO:0060720 - spongiotrophoblast cell proliferation (P)PMID:22562201ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2
updatedbyinstructorRatiujer,
Team Gene Simmons 2013
9BACL:I0BWH5GO:0044406 - adhesion of symbiont to host (P)PMID:22615573ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 3
requireschangesJohn Svoboda,
Team MSUM 2013
DROME:Q9VMH3GO:0007399 - nervous system development (P)PMID:22016536ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2
updatedbyinstructorKathrynmartin,
Team M Squared
HUMAN:ATG7GO:0051607 - defense response to virus (P)PMID:23290079ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2
updatedbyinstructorPallasbr,
Team KEGGstands at PubMed
MOUSE:FOSL2GO:0045893 - positive regulation of DNA-templated transcription (P)PMID:22585681ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2
updatedbyinstructorMill2034,
Team Its Miller Time
ARATH:AB1DGO:0016042 - lipid catabolic process (P)PMID:12065405ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 4
updatedbyinstructorSbutler,
Team Thundercats Ho!
HUMAN:KSYKGO:0045579 - positive regulation of B cell differentiation (P)PMID:18369315ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 5
updatedbyinstructorDetomas1,
Team Best in the NIT
ARATH:AMY3GO:0004556 - alpha-amylase activity (F)PMID:23019330ECO:0000314 direct assay evidence used in manual assertionchallengeC: 5
requireschangesAdeaton,
Team Jaberwocky
MOUSE:PIWL2GO:0043971 - obsolete histone H3-K18 acetylation (P)PMID:22110608ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1

0 annotations fixed by Schmi409

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