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PMID:15118832
| Citation |
Pak, FE, Gropper, S, Dai, WD, Havkin-Frenkel, D and Belanger, FC (2004) Characterization of a multifunctional methyltransferase from the orchid Vanilla planifolia. Plant Cell Rep. 22:959-66 |
|---|---|
| Abstract |
The final enzymatic step in the synthesis of the flavor compound vanillin (4-hydroxy-3-methoxybenzaldehyde) is believed to be methylation of 3,4-dihydroxybenzaldehyde. We have isolated and functionally characterized a cDNA that encodes a multifunctional methyltransferase from Vanilla planifolia tissue cultures that can catalyze the conversion of 3,4-dihydroxybenzaldehyde to vanillin, although 3,4-dihydroxybenzaldehyde is not the preferred substrate. The higher catalytic efficiency of the purified recombinant enzyme with the substrates caffeoyl aldehyde and 5-OH-coniferaldehyde, and its tissue distribution, suggest this methyltransferase may primarily function in lignin biosynthesis. However, since the enzyme characterized here does have 3,4-dihydroxybenzaldehyde-O-methyltransferase activity, it may be useful in engineering strategies for the synthesis of natural vanillin from alternate sources. |
| Links |
PubMed Online version:10.1007/s00299-004-0795-x |
| Keywords |
Amino Acid Sequence; Base Sequence; Cells, Cultured; Cloning, Molecular; Conserved Sequence; DNA Primers; DNA, Complementary/genetics; Medicago sativa/enzymology; Methyltransferases/chemistry; Methyltransferases/genetics; Methyltransferases/isolation & purification; Methyltransferases/metabolism; Molecular Sequence Data; Peptide Fragments/chemistry; Phylogeny; Polymerase Chain Reaction; Recombinant Proteins/metabolism; Sequence Alignment; Sequence Homology, Amino Acid; Substrate Specificity; Triticum/enzymology; Vanilla/enzymology |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0047763: caffeate O-methyltransferase activity |
ECO:0000021: |
F |
Table 1: Shows that culture crude protein extracts demonstrated caffeic acid O-methyltransferase activity. |
Missing: with/from | ||||
See also
References
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