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PMID:12620350
| Citation |
Frey, M, Huber, K, Park, WJ, Sicker, D, Lindberg, P, Meeley, RB, Simmons, CR, Yalpani, N and Gierl, A (2003) A 2-oxoglutarate-dependent dioxygenase is integrated in DIMBOA-biosynthesis. Phytochemistry 62:371-6 |
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| Abstract |
Benzoxazinoids are secondary metabolites of grasses that function as natural pesticides. While many steps of DIMBOA biosynthesis have been elucidated, the mechanism of the introduction of OCH(3)-group at the C-7 position was unknown. Inhibitor experiments in Triticum aestivum and Zea mays suggest that a 2-oxoglutarate-dependent dioxygenase catalyses the hydroxylation reaction at C-7. Cloning and reverse genetics analysis have identified the Bx6 gene that encodes this enzyme. Bx6 is located in the Bx-gene cluster of maize. |
| Links | |
| Keywords |
Amino Acid Sequence; Benzoxazines; Blotting, Northern; Chromosome Mapping; Cloning, Molecular; DNA Transposable Elements/genetics; Enzyme Inhibitors/pharmacology; Evolution, Molecular; Hydroxylation; Ketoglutaric Acids/metabolism; Methylation; Molecular Sequence Data; Mutation; Oxazines/metabolism; Oxygenases/antagonists & inhibitors; Oxygenases/genetics; Oxygenases/metabolism; Phenotype; Triticum/enzymology; Zea mays/enzymology; Zea mays/genetics |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0016706: 2-oxoglutarate-dependent dioxygenase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0016706: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors |
ECO:0000314: |
F |
Figure 2: Shows that the inhibition of 2-oxoglutarate-dependent dioxygenases significantly decreases the production of DIMBOA |
complete | ||||
See also
References
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