GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:12620350

From GONUTS
Jump to: navigation, search
Citation

Frey, M, Huber, K, Park, WJ, Sicker, D, Lindberg, P, Meeley, RB, Simmons, CR, Yalpani, N and Gierl, A (2003) A 2-oxoglutarate-dependent dioxygenase is integrated in DIMBOA-biosynthesis. Phytochemistry 62:371-6

Abstract

Benzoxazinoids are secondary metabolites of grasses that function as natural pesticides. While many steps of DIMBOA biosynthesis have been elucidated, the mechanism of the introduction of OCH(3)-group at the C-7 position was unknown. Inhibitor experiments in Triticum aestivum and Zea mays suggest that a 2-oxoglutarate-dependent dioxygenase catalyses the hydroxylation reaction at C-7. Cloning and reverse genetics analysis have identified the Bx6 gene that encodes this enzyme. Bx6 is located in the Bx-gene cluster of maize.

Links

PubMed

Keywords

Amino Acid Sequence; Benzoxazines; Blotting, Northern; Chromosome Mapping; Cloning, Molecular; DNA Transposable Elements/genetics; Enzyme Inhibitors/pharmacology; Evolution, Molecular; Hydroxylation; Ketoglutaric Acids/metabolism; Methylation; Molecular Sequence Data; Mutation; Oxazines/metabolism; Oxygenases/antagonists & inhibitors; Oxygenases/genetics; Oxygenases/metabolism; Phenotype; Triticum/enzymology; Zea mays/enzymology; Zea mays/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MAIZE:BX6

enables

GO:0016706: 2-oxoglutarate-dependent dioxygenase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MAIZE:BX6

GO:0016706: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors

ECO:0000314:

F

Figure 2: Shows that the inhibition of 2-oxoglutarate-dependent dioxygenases significantly decreases the production of DIMBOA

complete
CACAO 7359


See also

References

See Help:References for how to manage references in GONUTS.