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Category:Team Proteocritics

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesXANAU:DHLAHerdman1, Team Proteocritics2013-04-14 16:28:08 CDTGO:0018786 haloalkane dehalogenase activity (F)PMID:2687254ECO:0000316 genetic interaction evidence used in manual assertion

Fig. 3: It was found that DhlA is highly expressed in strains lacking dehalogenase activity and strains with impaired dicholoethane utilization abilities.

challenge
updatedbyinstructorPSEPU:Q9FD33Herdman1, Team Proteocritics2013-04-14 14:56:46 CDTGO:0016841 ammonia-lyase activity (F)PMID:11157934ECO:0000314 direct assay evidence used in manual assertion

Fig. 3A/3C shows that 4-hydroxylaminobenzoate is converted to PCA in cells with overexpressed PnbB.

challenge
updatedbyinstructorPSEPU:Q9FD36Herdman1, Team Proteocritics2013-04-14 14:23:25 CDTGO:0034556 nitrobenzoate nitroreductase activity (F)PMID:11157934ECO:0000314 direct assay evidence used in manual assertion

Fig. 3A/3B shows that in cells containing overexpressed PnbA, 4NBen is converted to 4-hydroxylaminobenzoate in the presence of NADH.

challenge
updatedbyinstructorPSEPS:O68391Herdman1, Team Proteocritics2013-04-14 13:56:51 CDTGO:0047102 aminomuconate-semialdehyde dehydrogenase activity (F)PMID:9721300ECO:0000314 direct assay evidence used in manual assertion

Fig. 2 indicates that 2-AMS was converted to 2-aminomuconate by AmnC. An absorbance maxima appears at A326 instead of A263 when AmnC is included.

challenge
updatedbyinstructorPSEUH:VDHSchmi409, Team Proteocritics2013-04-14 11:07:57 CDTGO:0050608 vanillin dehydrogenase activity (F)PMID:9098058ECO:0000314 direct assay evidence used in manual assertion

Figure 10: Shows that the recombinant E.coli XL1-Blue strains with vdh gene converted vanillin to vanillic acid.

challenge
acceptable9SPHN:G2IMC6Schmi409, Team Proteocritics2013-04-11 18:52:12 CDTGO:0050608 vanillin dehydrogenase activity (F)PMID:17928721ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4B: Shows that ligV-inactivation mutant (DLV) did not have significant growth on vanillin, yet the wild-type strain SYK-6 did have significant growth on vanillin

challenge
requireschangesVANPL:Q6Q796Schmi409, Team Proteocritics2013-04-11 14:36:10 CDTGO:0047763 caffeate O-methyltransferase activity (F)PMID:15118832ECO:0000021 physical interaction evidence

Table 1: Shows that culture crude protein extracts demonstrated caffeic acid O-methyltransferase activity.

challenge
updatedbyinstructorRHISY:A7BJ35Schmi409, Team Proteocritics2013-04-09 19:24:44 CDTGO:0016871 cycloartenol synthase activity (F)PMID:17617700ECO:0000314 direct assay evidence used in manual assertion

Figure 2: Shows that when the RsCAS gene was introduced into yeast, cycloartenols were produced

challenge
acceptableBACSU:GLKSchmi409, Team Proteocritics2013-04-08 13:24:46 CDTGO:0004340 glucokinase activity (F)PMID:9620975ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2: Shows that a mutant strain MD186 (affecting the glcK gene) has complete loss of glucose kinase activity

challenge
updatedbyinstructorMAIZE:BX6Schmi409, Team Proteocritics2013-04-08 09:31:25 CDTGO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (F)PMID:12620350ECO:0000314 direct assay evidence used in manual assertion

Figure 2: Shows that the inhibition of 2-oxoglutarate-dependent dioxygenases significantly decreases the production of DIMBOA

challenge
updatedbyinstructorROSHC:Q5I6B5Schmi409, Team Proteocritics2013-04-08 09:15:21 CDTGO:0004026 alcohol O-acetyltransferase activity (F)PMID:12692346ECO:0000314 direct assay evidence used in manual assertion

Figure 4: Shows that the RhAAT1 gene product catalyzed the production of geranyl acetate from gerniol and aceyl-CoA and that this was dependent on the presence of the RhAAT1 gene product (enzyme).

challenge
acceptableECOLI:PURUSchmi409, Team Proteocritics2013-03-29 01:56:31 CDTGO:0006164 purine nucleotide biosynthetic process (P)PMID:8226647ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2. Mutants of the purU gene, along with the wild-type strains of E.coli were grown on medium containing various amino acids. Mutants required purine or formate supplementation for growth indicating role of purU in de novo purine nucleotide biosynthesis.

challenge
updatedbyinstructorLACLL:ABIQHerdman1, Team Proteocritics2013-03-28 12:43:51 CDTGO:0004521 endoribonuclease activity (F)PMID:23279123ECO:0000314 direct assay evidence used in manual assertion

Figure 2B shows that in cells containing both abiQ and antiQ, antiQ RNA is cleaved resulting in multiple bands on agarose gel.

challenge

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