PMID:11157934

Citation	Hughes, MA and Williams, PA (2001) Cloning and characterization of the pnb genes, encoding enzymes for 4-nitrobenzoate catabolism in Pseudomonas putida TW3. J. Bacteriol. 183:1225-32
Abstract	Pseudomonas putida strain TW3 is able to metabolize 4-nitrotoluene via 4-nitrobenzoate (4NBen) and 3, 4-dihydroxybenzoic acid (protocatechuate [PCA]) to central metabolites. We have cloned, sequenced, and characterized a 6-kbp fragment of TW3 DNA which contains five genes, two of which encode the enzymes involved in the catabolism of 4NBen to PCA. In order, they encode a 4NBen reductase (PnbA) which is responsible for catalyzing the direct reduction of 4NBen to 4-hydroxylaminobenzoate with the oxidation of 2 mol of NADH per mol of 4NBen, a reductase-like enzyme (Orf1) which appears to have no function in the pathway, a regulator protein (PnbR) of the LysR family, a 4-hydroxylaminobenzoate lyase (PnbB) which catalyzes the conversion of 4-hydroxylaminobenzoate to PCA and ammonium, and a second lyase-like enzyme (Orf2) which is closely associated with pnbB but appears to have no function in the pathway. The central pnbR gene is transcribed in the opposite direction to the other four genes. These genes complete the characterization of the whole pathway of 4-nitrotoluene catabolism to the ring cleavage substrate PCA in P. putida strain TW3.
Links	PubMed PMC94995 Online version:10.1128/JB.183.4.1225-1232.2001
Keywords	Ammonia-Lyases/genetics; Bacterial Proteins/genetics; Biodegradation, Environmental; Cloning, Molecular; Genes, Bacterial; Genes, Regulator; Models, Genetic; Molecular Sequence Data; Multigene Family; Nitrobenzoates/metabolism; Oxidoreductases/genetics; Pseudomonas putida/enzymology; Pseudomonas putida/genetics; Sequence Analysis, DNA; Sequence Analysis, Protein
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
PSEPU:Q9FD36	enables	GO:0034556: nitrobenzoate nitroreductase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
PSEPU:Q9FD36		GO:0034556: nitrobenzoate nitroreductase activity	ECO:0000314:		F		Fig. 3A/3B shows that in cells containing overexpressed PnbA, 4NBen is converted to 4-hydroxylaminobenzoate in the presence of NADH.	complete CACAO 7956
PSEPU:Q9FD33		GO:0016841: ammonia-lyase activity	ECO:0000314:		F		Fig. 3A/3C shows that 4-hydroxylaminobenzoate is converted to PCA in cells with overexpressed PnbB.	complete CACAO 7982
PSEPU:Q9FD33	enables	GO:0016841: ammonia-lyase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.