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User:Herdman1

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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorLACLL:ABIQ2013-03-28 12:43:51 CDTGO:0004521 endoribonuclease activity (F)PMID:23279123ECO:0000314 direct assay evidence used in manual assertion

Figure 2B shows that in cells containing both abiQ and antiQ, antiQ RNA is cleaved resulting in multiple bands on agarose gel.

challenge
updatedbyinstructorPSEPS:O683912013-04-14 13:56:51 CDTGO:0047102 aminomuconate-semialdehyde dehydrogenase activity (F)PMID:9721300ECO:0000314 direct assay evidence used in manual assertion

Fig. 2 indicates that 2-AMS was converted to 2-aminomuconate by AmnC. An absorbance maxima appears at A326 instead of A263 when AmnC is included.

challenge
updatedbyinstructorPSEPU:Q9FD362013-04-14 14:23:25 CDTGO:0034556 nitrobenzoate nitroreductase activity (F)PMID:11157934ECO:0000314 direct assay evidence used in manual assertion

Fig. 3A/3B shows that in cells containing overexpressed PnbA, 4NBen is converted to 4-hydroxylaminobenzoate in the presence of NADH.

challenge
updatedbyinstructorPSEPU:Q9FD332013-04-14 14:56:46 CDTGO:0016841 ammonia-lyase activity (F)PMID:11157934ECO:0000314 direct assay evidence used in manual assertion

Fig. 3A/3C shows that 4-hydroxylaminobenzoate is converted to PCA in cells with overexpressed PnbB.

challenge
requireschangesXANAU:DHLA2013-04-14 16:28:08 CDTGO:0018786 haloalkane dehalogenase activity (F)PMID:2687254ECO:0000316 genetic interaction evidence used in manual assertion

Fig. 3: It was found that DhlA is highly expressed in strains lacking dehalogenase activity and strains with impaired dicholoethane utilization abilities.

challenge

acceptable:0
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Herdman1

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
updatedbyinstructorDylan.browning,
Team Phatty Acidz
BPPRD:VP16GO:0019012 - virion (C)PMID:15331712ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1

fixed by Herdman1
updatedbyinstructorFitzg128,
Team The Schmosbies
BACSU:FLHOGO:0071978 - bacterial-type flagellum-dependent swarming motility (P)PMID:22730131ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2
updatedbyinstructorFitzg128,
Team The Schmosbies
BACSU:FLIKGO:0071978 - bacterial-type flagellum-dependent swarming motility (P)PMID:22730131ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 3
updatedbyinstructorDrumhel2,
Team Fanny Pack Eradication
STRMU:Q8DWM3GO:0044011 - single-species biofilm formation on inanimate substrate (P)PMID:21078847ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1

fixed by Herdman1
updatedbyinstructorFinchcha,
Team We Love Nickelback
EMEND:E7EDM2GO:0072659 - protein localization to plasma membrane (P)PMID:22489878ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 3
updatedbyinstructorElkington,
Team Old Worm
CAEEL:O16720GO:0070551 - endoribonuclease activity, cleaving siRNA-paired mRNA (F)PMID:22904066ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
requireschangesMegan.Bosson,
Team Amino Acid
HUMAN:FOLR1GO:0005667 - transcription regulator complex (C)PMID:23243496ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2
updatedbyinstructorPriesko4,
Team ttKtt
RHILE:Q20DQ4GO:0008378 - galactosyltransferase activity (F)PMID:22110131ECO:0000314 direct assay evidence used in manual assertionchallengeC: 4

fixed by Herdman1

3 annotations fixed by Herdman1

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