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User:Finchcha

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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorMOUSE:PITX12013-03-30 23:33:13 CDTGO:0045892 negative regulation of transcription, DNA-dependent (P)PMID:21300782ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 shows that TERT expression levels are decreased when PITX1 expression is high. Figure 4 further details that TERT expression is controlled through PITX1 binding to the promoter region of TERT.

challenge
requireschangesPSEAE:Q9HTL42013-04-10 19:56:40 CDTGO:0001071 nucleic acid binding transcription factor activity (F)PMID:22275523ECO:0000314 direct assay evidence used in manual assertion

EMSA results in figure 2 prove OxyR binds to the promoter regions of several P. aerugenosa genes. Additionally, figure 3 shows that expression of dps, pvdS and trxB2 genes increased in the presence of OxyR activated by H2O2.

challenge
updatedbyinstructorCAMJU:B5LWZ92013-04-11 11:38:27 CDTGO:0006355 regulation of transcription, DNA-dependent (P)PMID:19502436ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 compares expression of ars operon genes in arsR deletion mutants with wild-type. Expression of ars operon genes is significantly increased in the deletion mutant proving the arsR gene acts as an ars operon repressor.

challenge
updatedbyinstructorEMEND:E7EDM22013-04-14 15:38:32 CDTGO:0072659 protein localization to plasma membrane (P)PMID:22489878ECO:0000315 mutant phenotype evidence used in manual assertion

"These phenotypes correlate with miss-routing of the YAT plasma membrane transporters AgtA (glutamate) and PrnB (proline) to the vacuole under conditions that, in the wild type, result in their delivery to the plasma membrane. "

challenge
acceptableEMEND:Q8TFN92013-04-14 16:47:14 CDTGO:0015200 methylammonium transmembrane transporter activity (F)PMID:12455974ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4C shows that meaA deletion mutants take up less methylammonium at 1mM final methylammonium concentration than wildtype A. nidulans suggesting meaA functions as an ammonium transporter.

challenge
acceptableEMEND:Q8TFN82013-04-14 16:51:49 CDTGO:0015200 methylammonium transmembrane transporter activity (F)PMID:12455974ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4C shows that mepA deletion mutants take up methylammonium at a lower rate than wildtype A. nidulans cells suggesting mepA's role as an ammonium transporter.

challenge
updatedbyinstructorEMEND:Q001712013-04-14 17:37:15 CDTGO:0045893 positive regulation of transcription, DNA-dependent (P)PMID:16467464ECO:0000315 mutant phenotype evidence used in manual assertion

Multiplex RT-PCR results in figure 6B shows that AreA deletion mutants transcribe less methylammonium transporter genes than wild-type suggesting that areA acts as a transcriptional activator for meaA and mepA.

challenge
updatedbyinstructorEMEND:Q400D82013-04-14 17:56:26 CDTGO:0015758 glucose transport (P)PMID:16418173ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 8.

challenge

acceptable:2
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Finchcha

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
updatedbyinstructorEmily.krummel,
Team Endoplasmic Reticulum
STRCO:Q9KYY6GO:0017000 - antibiotic biosynthetic process (P)PMID:23232715ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1
updatedbyinstructorMill2034,
Team Its Miller Time
ECOLI:CPXRGO:0045893 - positive regulation of transcription, DNA-templated (P)PMID:15743952ECO:0000314 direct assay evidence used in manual assertionchallengeC: 3
requireschangesMill2034,
Team Its Miller Time
?GO:0010629 - negative regulation of gene expression (Figure 8 shows that when a mutant for the gene is made, there is an increase in expression of the gene regulated by HDA6)PMID:22102827IMP: Inferred from Mutant Phenotype challengeC: 3
acceptableRaoneha,
Team Peacock
DROME:CHD1GO:0043044 - ATP-dependent chromatin remodeling (P)PMID:23533627ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1
updatedbyinstructorAndrew.james,
Team Gene Wilder
DROME:Q9Y1L3GO:0043044 - ATP-dependent chromatin remodeling (P)PMID:18250149ECO:0000314 direct assay evidence used in manual assertionchallengeC: 4
updatedbyinstructorAlemus,
Team Blot Blot Western Baby
HPV18:VE2GO:0044163 - host cytoskeleton (C)PMID:15020762ECO:0000314 direct assay evidence used in manual assertionchallengeC: 7

0 annotations fixed by Finchcha

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