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Category:Team Blot Blot Western Baby

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesHUMAN:RGS10Alemus, Team Blot Blot Western Baby2013-02-10 01:15:50 CSTGO:0005096 GTPase activator activity (F)PMID:10791963ECO:0000314 direct assay evidence used in manual assertion

Figure 3 shows RGS16 activates GFP by turning off Galpha and Gbeta quicker,as seen in the overlay (yellow) in flourescence picture shows the activation of GFP.

challenge
updatedbyinstructorHPV16:VE5Alemus, Team Blot Blot Western Baby2013-02-10 00:14:31 CSTGO:0033668 negative regulation by symbiont of host apoptotic process (P)PMID:11739687ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 shows a TUNNEL in parts A and B where E2 has kept HFKs from going under apoptosis after being exposed to UV. L(16E5)SN-infected keratinocytes were exposed to 0 (A) and 400 (B) J of UV light, the cells were stained with DAPI. this happens by E5 enhancing EGFR activation.

challenge
updatedbyinstructorHPV18:VE2Alemus, Team Blot Blot Western Baby2013-02-09 23:33:03 CSTGO:0044163 host cytoskeleton (C)PMID:15020762ECO:0000314 direct assay evidence used in manual assertion

Figure 1D shows that E2 protein from HPV 18 binds to mitotic spindles and helps pull apart DNA strands as shown in the 3rd overlap of DNA, E2 and mitotic spindles.

challenge
updatedbyinstructorHPV11:D7GMV3Alemus, Team Blot Blot Western Baby2013-02-09 23:24:37 CSTGO:0044163 host cytoskeleton (C)PMID:15020762ECO:0000314 direct assay evidence used in manual assertion

Figure 1A/B & Fig 2

challenge
updatedbyinstructorHPV16:VE2Alemus, Team Blot Blot Western Baby2013-02-09 23:12:33 CSTGO:0044163 host cytoskeleton (C)PMID:15020762ECO:0000314 direct assay evidence used in manual assertion

Figure 1 C shows that HPV 16 and E2 protein is involved in binding to mitotic spindles and pulling apart replicating DNA strands

challenge
updatedbyinstructor9HEPC:Q8V7L6Alemus, Team Blot Blot Western Baby2013-02-09 21:38:24 CSTGO:0052040 modulation by symbiont of host programmed cell death (P)PMID:19951438ECO:0000314 direct assay evidence used in manual assertion

Figure 5. Shows that zVAD inhibits all other proteins that use caspase to induce apoptosis in hepatic cells, but it only partially inhibits the core protein, meaning that the core protein induces apoptosis independently from caspase.

challenge
requireschangesNVN68:VP2Acapello, Team Blot Blot Western Baby2013-02-09 14:34:31 CSTGO:0043489 positive regulation of viral transcription (P)PMID:14557646ECO:0000314 direct assay evidence used in manual assertion

Figure 6 shows Western Blot analysis of VP1, VP2, and both VP1 and VP2 capsid protein expression after protease degradation. Demonstrates VP2 increased VP1 stability and protected VP1 from protease degradation.

challenge
requireschangesPSEPK:TOLBAcapello, Team Blot Blot Western Baby2013-02-09 13:52:23 CSTGO:0009279 cell outer membrane (C)PMID:10940016ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2: shows tolB is involved in outer membrane stability. A) shows normal P. putidia KT2440 gene E) shows tolB mutant gene.

challenge
updatedbyinstructorHPV18:VE6Acapello, Team Blot Blot Western Baby2013-02-09 13:11:02 CSTGO:0045892 negative regulation of transcription, DNA-dependent (P)PMID:21849431ECO:0000314 direct assay evidence used in manual assertion

Figure 5

challenge


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Pages in category "Team Blot Blot Western Baby"

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