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PMID:15020762

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Citation

Van Tine, BA, Dao, LD, Wu, SY, Sonbuchner, TM, Lin, BY, Zou, N, Chiang, CM, Broker, TR and Chow, LT (2004) Human papillomavirus (HPV) origin-binding protein associates with mitotic spindles to enable viral DNA partitioning. Proc. Natl. Acad. Sci. U.S.A. 101:4030-5

Abstract

Human papillomaviruses (HPVs) establish long-term infections in patients. The mechanism for extrachromosomal HPV DNA persistence in cycling cells is unknown. We show that HPV origin-containing plasmids partition as minichromosomes, attributable to an association of the viral origin recognition protein E2 with mitotic spindles. alpha-, beta-, and gamma-tubulins were pulled down with a tagged E2. The N-terminal transacting and C-terminal protein dimerization/DNA binding domains independently associated with the spindles. We suggest that this E2 property enables these viruses to establish persistence. Its implication for HPV oncogenesis is presented.

Links

PubMed PMC384690 Online version:10.1073/pnas.0306848101

Keywords

Centrosome/metabolism; DNA, Viral/metabolism; DNA-Binding Proteins/metabolism; Genes, Reporter; Genetic Vectors; Humans; Microtubule-Organizing Center/metabolism; Microtubules/metabolism; Mitotic Spindle Apparatus/metabolism; Papillomaviridae/metabolism; Plasmids; Protein Structure, Tertiary; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Viral Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HPV16:VE2

GO:0044163: host cytoskeleton

ECO:0000314:

C

Figure 1 C shows that HPV 16 and E2 protein is involved in binding to mitotic spindles and pulling apart replicating DNA strands

complete
CACAO 6495

HPV16:VE2

part_of

GO:0044163: host cytoskeleton

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HPV11:D7GMV3

GO:0044163: host cytoskeleton

ECO:0000314:

C

Figure 1A/B & Fig 2

complete
CACAO 6497

HPV11:D7GMV3

part_of

GO:0044163: host cytoskeleton

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HPV18:VE2

GO:0044163: host cytoskeleton

ECO:0000314:

C

Figure 1D shows that E2 protein from HPV 18 binds to mitotic spindles and helps pull apart DNA strands as shown in the 3rd overlap of DNA, E2 and mitotic spindles.

complete
CACAO 6499

HPV18:VE2

part_of

GO:0044163: host cytoskeleton

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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