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PMID:22489878

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Citation

Apostolaki, A, Harispe, L, Calcagno-Pizarelli, AM, Vangelatos, I, Sophianopoulou, V, Arst, HN Jr, Peñalva, MA, Amillis, S and Scazzocchio, C (2012) Aspergillus nidulans CkiA is an essential casein kinase I required for delivery of amino acid transporters to the plasma membrane. Mol. Microbiol. 84:530-49

Abstract

Type I casein kinases are highly conserved among Eukaryotes. Of the two Aspergillus nidulans casein kinases I, CkiA is related to the δ/ε mammalian kinases and to Saccharomyces cerevisiae Hrr25p. CkiA is essential. Three recessive ckiA mutations leading to single residue substitutions, and downregulation using a repressible promoter, result in partial loss-of-function, which leads to a pleiotropic defect in amino acid utilization and resistance to toxic amino acid analogues. These phenotypes correlate with miss-routing of the YAT plasma membrane transporters AgtA (glutamate) and PrnB (proline) to the vacuole under conditions that, in the wild type, result in their delivery to the plasma membrane. Miss-routing to the vacuole and subsequent transporter degradation results in a major deficiency in the uptake of the corresponding amino acids that underlies the inability of the mutant strains to catabolize them. Our findings may have important implications for understanding how CkiA, Hrr25p and other fungal orthologues regulate the directionality of transport at the ER-Golgi interface.

Links

PubMed PMC3491690 Online version:10.1111/j.1365-2958.2012.08042.x

Keywords

Amino Acid Sequence; Amino Acid Transport Systems/genetics; Amino Acid Transport Systems/metabolism; Aspergillus nidulans/chemistry; Aspergillus nidulans/enzymology; Aspergillus nidulans/genetics; Aspergillus nidulans/metabolism; Biological Transport; Casein Kinase I/chemistry; Casein Kinase I/genetics; Casein Kinase I/metabolism; Cell Membrane/genetics; Cell Membrane/metabolism; Fungal Proteins/chemistry; Fungal Proteins/genetics; Fungal Proteins/metabolism; Glutamic Acid/metabolism; Molecular Sequence Data; Proline/metabolism; Protein Transport; Sequence Homology, Amino Acid

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

EMENI:C8V2E0

located_in

GO:0000324: fungal-type vacuole

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

EMENI:C8V2E0

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

EMENI:C8V7X8

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

EMENI:C8V7X8

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

EMEND:E7EDM2

involved_in

GO:0072659: protein localization to plasma membrane

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

EMEND:E7EDM2

GO:0072659: protein localization to plasma membrane

ECO:0000315:

P

"These phenotypes correlate with miss-routing of the YAT plasma membrane transporters AgtA (glutamate) and PrnB (proline) to the vacuole under conditions that, in the wild type, result in their delivery to the plasma membrane. "

complete
CACAO 8009


See also

References

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