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PMID:16418173

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Citation

Forment, JV, Flipphi, M, Ramón, D, Ventura, L and Maccabe, AP (2006) Identification of the mstE gene encoding a glucose-inducible, low affinity glucose transporter in Aspergillus nidulans. J. Biol. Chem. 281:8339-46

Abstract

The mstE gene encoding a low affinity glucose transporter active during the germination of Aspergillus nidulans conidia on glucose medium has been identified. mstE expression also occurs in hyphae, is induced in the presence of other repressing carbon sources besides glucose, and is dependent on the function of the transcriptional repressor CreA. The expression of MstE and its subcellular distribution have been studied using a MstE-sGFP fusion protein. Concordant with data on mstE expression, MstE-sGFP is synthesized in the presence of repressing carbon sources, and fluorescence at the periphery of conidia and hyphae is consistent with MstE location in the plasma membrane. Deletion of mstE has no morphological phenotype but results in the absence of low affinity glucose uptake kinetics, the latter being substituted by a high affinity system.

Links

PubMed Online version:10.1074/jbc.M508198200

Keywords

Amino Acid Sequence; Aspergillus nidulans/genetics; Blotting, Northern; Cell Membrane/metabolism; DNA, Complementary/genetics; DNA, Complementary/isolation & purification; Databases, Factual; Expressed Sequence Tags; Fungal Proteins; Gene Deletion; Gene Expression Regulation, Fungal; Genes, Fungal; Glucose/pharmacokinetics; Glucose/pharmacology; Green Fluorescent Proteins/metabolism; Hyphae/metabolism; Kinetics; Microscopy, Fluorescence; Molecular Sequence Data; Monosaccharide Transport Proteins/chemistry; Monosaccharide Transport Proteins/genetics; Monosaccharide Transport Proteins/metabolism; Phylogeny; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/metabolism; Repressor Proteins/metabolism; Spores, Fungal/metabolism; Spores, Fungal/physiology; Substrate Specificity

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

EMENI:Q5B0S0

enables

GO:0015578: mannose transmembrane transporter activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

EMENI:Q5B0S0

enables

GO:0005355: glucose transmembrane transporter activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

EMENI:Q5B0S0

enables

GO:0005355: glucose transmembrane transporter activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

EMENI:Q5B0S0

acts_upstream_of_or_within

GO:0034219: carbohydrate transmembrane transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

EMENI:Q5B0S0

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

EMENI:Q5B0S0

located_in

GO:0005773: vacuole

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

EMENI:Q5B0S0

acts_upstream_of_or_within

GO:1904659: glucose transmembrane transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

EMEND:Q400D8

involved_in

GO:1904659: glucose transmembrane transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

EMEND:Q400D8

GO:0015758: glucose transport

ECO:0000315:

P

Fig 8.

complete
CACAO 8114


See also

References

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