PMID:16467464

Citation	Monahan, BJ, Askin, MC, Hynes, MJ and Davis, MA (2006) Differential expression of Aspergillus nidulans ammonium permease genes is regulated by GATA transcription factor AreA. Eukaryotic Cell 5:226-37
Abstract	The movement of ammonium across biological membranes is mediated in both prokaryotes and eukaryotes by ammonium transport proteins (AMT/MEP) that constitute a family of related sequences. We have previously identified two ammonium permeases in Aspergillus nidulans, encoded by the meaA and mepA genes. Here we show that meaA is expressed in the presence of ammonium, consistent with the function of MeaA as the main ammonium transporter required for optimal growth on ammonium as a nitrogen source. In contrast, mepA, which encodes a high-affinity ammonium permease, is expressed only under nitrogen-limiting or starvation conditions. We have identified two additional AMT/MEP-like genes in A. nidulans, namely, mepB, which encodes a second high-affinity ammonium transporter expressed only in response to complete nitrogen starvation, and mepC, which is expressed at low levels under all nitrogen conditions. The MepC gene product is more divergent than the other A. nidulans AMT/MEP proteins and is not thought to significantly contribute to ammonium uptake under normal conditions. Remarkably, the expression of each AMT/MEP gene under all nitrogen conditions is regulated by the global nitrogen regulatory GATA factor AreA. Therefore, AreA is also active under nitrogen-sufficient conditions, along with its established role as a transcriptional activator in response to nitrogen limitation.
Links	PubMed PMC1405890 Online version:10.1128/EC.5.2.226-237.2006
Keywords	Amino Acid Sequence; Aspergillus nidulans/cytology; Aspergillus nidulans/drug effects; Aspergillus nidulans/genetics; DNA-Binding Proteins/metabolism; Fungal Proteins/metabolism; GATA Transcription Factors/metabolism; Gene Deletion; Gene Expression Profiling; Gene Expression Regulation, Fungal; Genes, Fungal/genetics; Membrane Transport Proteins/chemistry; Membrane Transport Proteins/genetics; Membrane Transport Proteins/metabolism; Molecular Sequence Data; Quaternary Ammonium Compounds/metabolism; Quaternary Ammonium Compounds/pharmacology; Sequence Homology, Amino Acid; Transcription Factors/metabolism
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
EMEND:Q00171		GO:0045893: positive regulation of transcription, DNA-dependent	ECO:0000315:		P		Multiplex RT-PCR results in figure 6B shows that AreA deletion mutants transcribe less methylammonium transporter genes than wild-type suggesting that areA acts as a transcriptional activator for meaA and mepA.	complete CACAO 8103
EMEND:Q00171	involved_in	GO:0045893: positive regulation of transcription, DNA-templated	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.