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PMID:18250149

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Citation

Murawska, M, Kunert, N, van Vugt, J, Längst, G, Kremmer, E, Logie, C and Brehm, A (2008) dCHD3, a novel ATP-dependent chromatin remodeler associated with sites of active transcription. Mol. Cell. Biol. 28:2745-57

Abstract

ATP-dependent chromatin remodelers of the CHD family play important roles during differentiation and development. Three CHD proteins, dMi-2, dChd1, and Kismet, have been described for Drosophila melanogaster. Here, we study dCHD3, a novel member of the CHD family. dCHD3 is related in sequence to dMi-2 but lacks several domains implicated in dMi-2 function. We demonstrate that dCHD3 is a nuclear protein and that expression is tightly regulated during fly development. Recombinant dCHD3 remodels mono- and polynucleosomes in an ATP-dependent manner in vitro. Its chromodomains are critical for nucleosome binding and remodeling. Unlike dMi-2, dCHD3 exists as a monomer. Nevertheless, both proteins colocalize with RNA polymerase II to actively transcribed regions on polytene chromosomes, suggesting that both remodelers participate in the process of transcription.

Links

PubMed PMC2293103 Online version:10.1128/MCB.01839-07

Keywords

Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Adenosine Triphosphate/metabolism; Animals; Autoantigens/genetics; Autoantigens/metabolism; Chromatin Assembly and Disassembly/genetics; Chromosomes/genetics; DNA/metabolism; DNA Helicases/genetics; DNA Helicases/metabolism; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/embryology; Drosophila melanogaster/genetics; Drosophila melanogaster/growth & development; Drosophila melanogaster/metabolism; Gene Expression Regulation, Developmental; Histones/metabolism; Nucleosomes/enzymology; Phylogeny; Protein Binding; Transcription, Genetic/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:CHD3

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:CHD3

located_in

GO:0035327: transcriptionally active chromatin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:CHD3

enables

GO:0031491: nucleosome binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:CHD3

enables

GO:0016887: ATP hydrolysis activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:CHD3

enables

GO:0003677: DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:CHD3

enables

GO:0140658: ATP-dependent chromatin remodeler activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:CHD3

involved_in

GO:0042766: nucleosome mobilization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:CHDM

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:CHDM

located_in

GO:0035327: transcriptionally active chromatin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:CHDM

enables

GO:0016887: ATP hydrolysis activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:CHDM

enables

GO:0140658: ATP-dependent chromatin remodeler activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:CHDM

enables

GO:0031491: nucleosome binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:CHDM

part_of

GO:0035327: transcriptionally active chromatin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:CHDM

involved_in

GO:0042766: nucleosome mobilization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:CHDM

enables

GO:0003677: DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:CHDM

enables

GO:0070615: nucleosome-dependent ATPase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:CHDM

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9Y1L3

GO:0140658: ATP-dependent chromatin remodeler activity

ECO:0000314: direct assay evidence used in manual assertion

F

Figure 3 & 4.

GO term changed per Ticket-3833 ~~~~

complete
CACAO 7133

DROME:Q9Y1L3

involved_in

GO:0140658: ATP-dependent chromatin remodeler activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

GO term changed per Ticket-3833 ~~~~

complete


See also

References

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