User:Fitzg128

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
updatedbyinstructor	BACSU:FLIK	2013-03-31 11:39:29 CDT	GO:0071978 bacterial-type flagellar swarming motility (P)	PMID:22730131	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig. 1C shows that a mutation in fliK abolished the swarming motility observed in WT cells.	challenge
updatedbyinstructor	BACSU:FLHO	2013-03-31 11:47:17 CDT	GO:0071978 bacterial-type flagellar swarming motility (P)	PMID:22730131	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 1.C. Mutations were engineered into fLHO and expression of mutant reduced swarm motility.	challenge
requireschanges	BACSU:FLGM	2013-03-31 12:06:20 CDT	GO:0043064 flagellum organization (P)	PMID:22730131	ECO:0000315 mutant phenotype evidence used in manual assertion	Figures 5 A and B. FlgM shows higher miller unit activity compared to control	challenge
acceptable	BACSU:CHIS	2013-04-14 13:18:23 CDT	GO:0016977 chitosanase activity (F)	PMID:23065029	ECO:0000314 direct assay evidence used in manual assertion	Figure 3. Two strains of the Fungus Rhizopus mycelium were tested in a control and experimental group. The experimental groups with CSNV26 expressed had deformed the chitosan cell walls of the fungi.	challenge
acceptable	BACSU:MRPA	2013-04-14 13:47:32 CDT	GO:0015385 sodium:hydrogen antiporter activity (F)	PMID:20509166	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 5. Panel A: MprA null mutants are unable to grow in the sodium rich environments compared to both wild type and cells with MprA placed back into the cells via a plasmid.	challenge
acceptable	BACSU:MRPD	2013-04-14 13:50:23 CDT	GO:0015385 sodium:hydrogen antiporter activity (F)	PMID:20509166	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 5. Panel B: MprD null mutants are unable to grow in the sodium rich environments compared to both wild type cells and cells with MprD placed back into the cells via a plasmid.	challenge
requireschanges	FLAJ1:A1E5U4	2013-04-14 14:29:17 CDT	GO:0048870 cell motility (P)	PMID:3021240	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 5: Tracks illustrating the movements of the cells shown in panels A to I over a 60-s period were obtained by superimposing individual digital video frames over Palmer cell slides showed decreased motility in SprD as compared to the wild type, which was shown in portion F of figure 5.	challenge
requireschanges	FLAJ1:A1E5U3	2013-04-14 14:32:50 CDT	GO:0048870 cell motility (P)	PMID:3021240	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 5. Portion B. Video showing tracts of cell movement compared to the wild phenotype, show decreased motility in cells with mutations in SprC.	challenge
acceptable	FLAJ1:GLDH	2013-04-14 14:44:19 CDT	GO:0071976 cell gliding (P)	PMID:14594839	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2. Shows that wild type strain has gliding motility (A). Mutants do not (B). Mutants treated with plasmid containg GldH recover motility(C). Plasmids without GldH do not recover motility (D).	challenge
updatedbyinstructor	FLAJ1:GLDH	2013-04-14 14:51:04 CDT	GO:0006032 chitin catabolic process (P)	PMID:14594839	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 5: Portion H-/+ Mutations in GldH show absence of chitinase activity, compared to when glDH mutants are co-expressed with plasmids containing GldH.	challenge
acceptable	FLAJ1:A5FJM9	2013-04-14 15:03:39 CDT	GO:0071976 cell gliding (P)	PMID:15805509	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2. (A-E) (A) Wild-type F. johnsoniae UW101 without gldJ. (B) Wild-type cells gldJ. (C) gldJ mutant UW102-48 without gldJ. (D) UW102-48 complemented with pMM313 that carries gldJ. (E) Wild-type cells, which carries a truncated version of gldJ.	challenge
unacceptable	EBOZM:VP35	2013-04-14 15:23:29 CDT	GO:0003723 RNA binding (F)	PMID:19233284	ECO:0000314 direct assay evidence used in manual assertion	VP35 binds to a polyCG dsRNA linked to agarose beads, while it does not interact with the agarose beads without RNA (Fig. 4A, lanes 1 and 2).	challenge

acceptable:5
unacceptable:1
requires_changes:3
flagged:0

Annotations challenged by Fitzg128

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

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