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EBOZM:VP35

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Species (Taxon ID) Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus). (128952)
Gene Name(s) VP35
Protein Name(s) Polymerase cofactor VP35
External Links
UniProt Q05127
EMBL X61274
L11365
AF086833
AF272001
AY142960
AF499101
RefSeq NP_066244.1
PDB 3FKE
3L25
3L26
3L27
3L28
3L29
4IBB
4IBC
4IBD
4IBE
4IBF
4IBG
4IBI
4IBJ
4IBK
4IJE
4IJF
PDBsum 3FKE
3L25
3L26
3L27
3L28
3L29
4IBB
4IBC
4IBD
4IBE
4IBF
4IBG
4IBI
4IBJ
4IBK
4IJE
4IJF
DIP DIP-48771N
IntAct Q05127
GeneID 911827
EvolutionaryTrace Q05127
Proteomes UP000007209
GO GO:0030430
GO:0030529
GO:0019013
GO:0003723
GO:0030683
GO:0010629
GO:0060965
GO:0039505
GO:0046775
GO:0039724
GO:0039549
GO:0039558
GO:0039723
GO:0039722
GO:0039501
GO:0044414
GO:0006351
InterPro IPR002953
Pfam PF02097
PIRSF PIRSF018326
PRINTS PR01240
PROSITE PS51735

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0046775

suppression by virus of host cytokine production

PMID:19828757[1]

ECO:0000314

P

Fig. 3 and Figs. 6DEF

complete
CACAO 2409

GO:0039505

suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II

PMID:19828757[1]

ECO:0000315

P

Fig. 2 and Fig. 4

complete
CACAO 2410

GO:0050868

negative regulation of T cell activation

PMID:19828757[1]

ECO:0000315

P

Fig. 5

complete
CACAO 2411

GO:0030683

evasion or tolerance by virus of host immune response

PMID:19828757[1]

ECO:0000314

P

Fig. 6 Inhibits dendritic cell maturation

complete
CACAO 2412

GO:0044414

suppression of host defenses

PMID:21228243[2]

ECO:0000315

P

Fig. 2 Suppression of siRNA

complete
CACAO 2416

GO:0039613

suppression by virus of host protein phosphorylation

PMID:12829834[3]

ECO:0000314

P

Figure 4 shows VP35 blocks dimerization and phosphorylation of IRF-3 in response to SeV infection.

complete
CACAO 5213

GO:0010629

negative regulation of gene expression

PMID:12829834[3]

ECO:0000314

P

Figure 2:The Ebola virus VP35 protein can block virus-induced, IRF-3-dependent gene expression.

complete
CACAO 5359

GO:0046983

protein dimerization activity

PMID:12829834[3]

ECO:0000314

F

Figure 4: VP35 blocks dimerization

complete
CACAO 5360

GO:0039580

suppression by virus of host PKR activity

PMID:17065211[4]

ECO:0000314

P

Figure 4: PKR activity is inhibited by VP35 (present in MC0201)

complete

GO:0060965

negative regulation of gene silencing by miRNA

PMID:22238300[5]

ECO:0000314

P

Figure 1: 1A shows a Luciferase assay of the expression level of the target sequence (miR30) without the presence of VP35. Its expression is successfully silenced by the host defense. 1E the expression of miR30 with addition of the VP35 plasmid. Silencing was suppressed and expression was of the target sequence was enhanced.

complete
CACAO 6380

GO:0030529

ribonucleoprotein complex

PMID:19041915[6]

ECO:0000314

C

Figure 3: VP35 was coexpressed in a plasmid with known Ribonucleoprotein complex components NP and L. Antisera against all components were used in two-color co-immunofluorescence. This figure shows that VP35 was able to be co-localized with both known RNP components, forming a bridge.

complete
CACAO 6381

GO:0019013

viral nucleocapsid

PMID:12191476[7]

ECO:0000314

C

Figure 2B shows the presence of VP 35 in the density centrifugation and Western Blot of the viral nucleocapsid.

complete
CACAO 6459

GO:0033235

positive regulation of protein sumoylation

PMID:19557165[8]

ECO:0000315

P

Figure 7 shows that VP35 promotes SUMOylation of IRF7 positive regulation of the SUMO ligase, PIAS-1.

complete
CACAO 6882

GO:0003690

double-stranded DNA binding

PMID:16698997[9]

ECO:0000315

F

Figure 1. All of these results show VP35s ability to bind dsDNA. An in vitro "pull-down" assay was used. Cell lysates were prepared and incubated with pIC-Sepharose and then visualized by Western blotting.

complete
CACAO 6914

GO:0039501

suppression by virus of host type I interferon production

PMID:16698997[9]

ECO:0000315

P

Figure 2. The abilities of wild-type VP35, R312A, and K309A to inhibit the SeV-induced activation of an IFN-Beta promoter reporter plasmid were compared. Western blotting was used to demonstrate the ability of VP35 to regulate IFN-Beta in Figure 2A, while Figure B shows the results from a panel overlay onto Vero cells in a 96-well plate and subsequent fluorescence.

complete
CACAO 6915

GO:0003723

RNA binding

PMID:19233284[10]

ECO:0000314

F

VP35 binds to a polyCG dsRNA linked to agarose beads, while it does not interact with the agarose beads without RNA (Fig. 4A, lanes 1 and 2).

complete
CACAO 8000

involved_in

GO:0060965

negative regulation of gene silencing by miRNA

PMID:22238300[5]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0046775

suppression by virus of host cytokine production

PMID:19828757[1]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0044414

suppression of host defenses

PMID:21228243[2]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0033235

positive regulation of protein sumoylation

PMID:19557165[8]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0039613

suppression by virus of host protein phosphorylation

PMID:12829834[3]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0039505

suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II

PMID:19828757[1]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0039501

suppression by virus of host type I interferon production

PMID:16698997[9]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0030683

evasion or tolerance by virus of host immune response

PMID:19828757[1]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

part_of

GO:0019013

viral nucleocapsid

PMID:12191476[7]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

involved_in

GO:0010629

negative regulation of gene expression

PMID:12829834[3]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

part_of

GO:0030430

host cell cytoplasm

GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-1035
UniProtKB-SubCell:SL-0381

C

Seeded From UniProt

complete

involved_in

GO:0030683

evasion or tolerance by virus of host immune response

GO_REF:0000037
GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0922
UniProtKB-KW:KW-0899

P

Seeded From UniProt

complete

involved_in

GO:0039503

suppression by virus of host innate immune response

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-1090

P

Seeded From UniProt

complete

involved_in

GO:0039722

suppression by virus of host toll-like receptor signaling pathway

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-1225

P

Seeded From UniProt

complete

involved_in

GO:0016032

viral process

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0945

P

Seeded From UniProt

complete

part_of

GO:0019012

virion

GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0946
UniProtKB-SubCell:SL-0274

C

Seeded From UniProt

complete

involved_in

GO:0039723

suppression by virus of host TBK1 activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-1223

P

Seeded From UniProt

complete

involved_in

GO:0039557

suppression by virus of host IRF7 activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-1093

P

Seeded From UniProt

complete

enables

GO:0003723

RNA binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0694

F

Seeded From UniProt

complete

involved_in

GO:0039724

suppression by virus of host IKBKE activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-1224

P

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Jin, H et al. (2010) The VP35 protein of Ebola virus impairs dendritic cell maturation induced by virus and lipopolysaccharide. J. Gen. Virol. 91 352-61 PubMed GONUTS page
  2. 2.0 2.1 Fabozzi, G et al. (2011) Ebolavirus proteins suppress the effects of small interfering RNA by direct interaction with the mammalian RNA interference pathway. J. Virol. 85 2512-23 PubMed GONUTS page
  3. 3.0 3.1 3.2 3.3 3.4 Basler, CF et al. (2003) The Ebola virus VP35 protein inhibits activation of interferon regulatory factor 3. J. Virol. 77 7945-56 PubMed GONUTS page
  4. Feng, Z et al. (2007) The VP35 protein of Ebola virus inhibits the antiviral effect mediated by double-stranded RNA-dependent protein kinase PKR. J. Virol. 81 182-92 PubMed GONUTS page
  5. 5.0 5.1 Zhu, Y et al. (2012) Characterization of the RNA silencing suppression activity of the Ebola virus VP35 protein in plants and mammalian cells. J. Virol. 86 3038-49 PubMed GONUTS page
  6. Groseth, A et al. (2009) The Ebola virus ribonucleoprotein complex: a novel VP30-L interaction identified. Virus Res. 140 8-14 PubMed GONUTS page
  7. 7.0 7.1 Huang, Y et al. (2002) The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein. Mol. Cell 10 307-16 PubMed GONUTS page
  8. 8.0 8.1 Chang, TH et al. (2009) Ebola Zaire virus blocks type I interferon production by exploiting the host SUMO modification machinery. PLoS Pathog. 5 e1000493 PubMed GONUTS page
  9. 9.0 9.1 9.2 Cárdenas, WB et al. (2006) Ebola virus VP35 protein binds double-stranded RNA and inhibits alpha/beta interferon production induced by RIG-I signaling. J. Virol. 80 5168-78 PubMed GONUTS page
  10. Zinzula, L et al. (2009) Purification and functional characterization of the full length recombinant Ebola virus VP35 protein expressed in E. coli. Protein Expr. Purif. 66 113-9 PubMed GONUTS page