EBOZM:VP35

Species (Taxon ID)	Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus). (128952)
Gene Name(s)	VP35
Protein Name(s)	Polymerase cofactor VP35
External Links
UniProt	Q05127
EMBL	X61274 L11365 AF086833 AF272001 AY142960 AF499101
RefSeq	NP_066244.1
PDB	3FKE 3L25 3L26 3L27 3L28 3L29 4IBB 4IBC 4IBD 4IBE 4IBF 4IBG 4IBI 4IBJ 4IBK 4IJE 4IJF
PDBsum	3FKE 3L25 3L26 3L27 3L28 3L29 4IBB 4IBC 4IBD 4IBE 4IBF 4IBG 4IBI 4IBJ 4IBK 4IJE 4IJF
DIP	DIP-48771N
IntAct	Q05127
GeneID	911827
EvolutionaryTrace	Q05127
Proteomes	UP000007209
GO	GO:0030430 GO:0030529 GO:0019013 GO:0003723 GO:0030683 GO:0010629 GO:0060965 GO:0039505 GO:0046775 GO:0039724 GO:0039549 GO:0039558 GO:0039723 GO:0039722 GO:0039501 GO:0044414 GO:0006351
InterPro	IPR002953
Pfam	PF02097
PIRSF	PIRSF018326
PRINTS	PR01240
PROSITE	PS51735

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Notes	Status
	GO:0046775	suppression by virus of host cytokine production	PMID:19828757^[1]	ECO:0000314			P	Fig. 3 and Figs. 6DEF	complete CACAO 2409
	GO:0039505	suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II	PMID:19828757^[1]	ECO:0000315			P	Fig. 2 and Fig. 4	complete CACAO 2410
	GO:0050868	negative regulation of T cell activation	PMID:19828757^[1]	ECO:0000315			P	Fig. 5	complete CACAO 2411
	GO:0030683	evasion or tolerance by virus of host immune response	PMID:19828757^[1]	ECO:0000314			P	Fig. 6 Inhibits dendritic cell maturation	complete CACAO 2412
	GO:0044414	suppression of host defenses	PMID:21228243^[2]	ECO:0000315			P	Fig. 2 Suppression of siRNA	complete CACAO 2416
	GO:0039613	suppression by virus of host protein phosphorylation	PMID:12829834^[3]	ECO:0000314			P	Figure 4 shows VP35 blocks dimerization and phosphorylation of IRF-3 in response to SeV infection.	complete CACAO 5213
	GO:0010629	negative regulation of gene expression	PMID:12829834^[3]	ECO:0000314			P	Figure 2:The Ebola virus VP35 protein can block virus-induced, IRF-3-dependent gene expression.	complete CACAO 5359
	GO:0046983	protein dimerization activity	PMID:12829834^[3]	ECO:0000314			F	Figure 4: VP35 blocks dimerization	complete CACAO 5360
	GO:0039580	suppression by virus of host PKR activity	PMID:17065211^[4]	ECO:0000314			P	Figure 4: PKR activity is inhibited by VP35 (present in MC0201)	complete
	GO:0060965	negative regulation of gene silencing by miRNA	PMID:22238300^[5]	ECO:0000314			P	Figure 1: 1A shows a Luciferase assay of the expression level of the target sequence (miR30) without the presence of VP35. Its expression is successfully silenced by the host defense. 1E the expression of miR30 with addition of the VP35 plasmid. Silencing was suppressed and expression was of the target sequence was enhanced.	complete CACAO 6380
	GO:0030529	ribonucleoprotein complex	PMID:19041915^[6]	ECO:0000314			C	Figure 3: VP35 was coexpressed in a plasmid with known Ribonucleoprotein complex components NP and L. Antisera against all components were used in two-color co-immunofluorescence. This figure shows that VP35 was able to be co-localized with both known RNP components, forming a bridge.	complete CACAO 6381
	GO:0019013	viral nucleocapsid	PMID:12191476^[7]	ECO:0000314			C	Figure 2B shows the presence of VP 35 in the density centrifugation and Western Blot of the viral nucleocapsid.	complete CACAO 6459
	GO:0033235	positive regulation of protein sumoylation	PMID:19557165^[8]	ECO:0000315			P	Figure 7 shows that VP35 promotes SUMOylation of IRF7 positive regulation of the SUMO ligase, PIAS-1.	complete CACAO 6882
	GO:0003690	double-stranded DNA binding	PMID:16698997^[9]	ECO:0000315			F	Figure 1. All of these results show VP35s ability to bind dsDNA. An in vitro "pull-down" assay was used. Cell lysates were prepared and incubated with pIC-Sepharose and then visualized by Western blotting.	complete CACAO 6914
	GO:0039501	suppression by virus of host type I interferon production	PMID:16698997^[9]	ECO:0000315			P	Figure 2. The abilities of wild-type VP35, R312A, and K309A to inhibit the SeV-induced activation of an IFN-Beta promoter reporter plasmid were compared. Western blotting was used to demonstrate the ability of VP35 to regulate IFN-Beta in Figure 2A, while Figure B shows the results from a panel overlay onto Vero cells in a 96-well plate and subsequent fluorescence.	complete CACAO 6915
	GO:0003723	RNA binding	PMID:19233284^[10]	ECO:0000314			F	VP35 binds to a polyCG dsRNA linked to agarose beads, while it does not interact with the agarose beads without RNA (Fig. 4A, lanes 1 and 2).	complete CACAO 8000
involved_in	GO:0060965	negative regulation of gene silencing by miRNA	PMID:22238300^[5]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0046775	suppression by virus of host cytokine production	PMID:19828757^[1]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0044414	suppression of host defenses	PMID:21228243^[2]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0033235	positive regulation of protein sumoylation	PMID:19557165^[8]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0039505	suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II	PMID:19828757^[1]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0039501	suppression by virus of host type I interferon production	PMID:16698997^[9]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0030683	evasion or tolerance by virus of host immune response	PMID:19828757^[1]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
part_of	GO:0019013	viral nucleocapsid	PMID:12191476^[7]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
involved_in	GO:0010629	negative regulation of gene expression	PMID:12829834^[3]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
part_of	GO:0030430	host cell cytoplasm	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1035 UniProtKB-SubCell:SL-0381	C	Seeded From UniProt	complete
involved_in	GO:0030683	evasion or tolerance by virus of host immune response	GO_REF:0000037 GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0922 UniProtKB-KW:KW-0899	P	Seeded From UniProt	complete
involved_in	GO:0039503	suppression by virus of host innate immune response	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1090	P	Seeded From UniProt	complete
involved_in	GO:0039722	suppression by virus of host toll-like receptor signaling pathway	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1225	P	Seeded From UniProt	complete
involved_in	GO:0016032	viral process	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0945	P	Seeded From UniProt	complete
part_of	GO:0019012	virion	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0946 UniProtKB-SubCell:SL-0274	C	Seeded From UniProt	complete
involved_in	GO:0039723	suppression by virus of host TBK1 activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1223	P	Seeded From UniProt	complete
involved_in	GO:0039557	suppression by virus of host IRF7 activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1093	P	Seeded From UniProt	complete
enables	GO:0003723	RNA binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0694	F	Seeded From UniProt	complete
involved_in	GO:0039724	suppression by virus of host IKBKE activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1224	P	Seeded From UniProt	complete
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Notes

References

See Help:References for how to manage references in GONUTS.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 Jin, H et al. (2010) The VP35 protein of Ebola virus impairs dendritic cell maturation induced by virus and lipopolysaccharide. J. Gen. Virol. 91 352-61 PubMed GONUTS page
↑ ^2.0 ^2.1 Fabozzi, G et al. (2011) Ebolavirus proteins suppress the effects of small interfering RNA by direct interaction with the mammalian RNA interference pathway. J. Virol. 85 2512-23 PubMed GONUTS page
↑ ^3.0 ^3.1 ^3.2 ^3.3 Basler, CF et al. (2003) The Ebola virus VP35 protein inhibits activation of interferon regulatory factor 3. J. Virol. 77 7945-56 PubMed GONUTS page
↑ Feng, Z et al. (2007) The VP35 protein of Ebola virus inhibits the antiviral effect mediated by double-stranded RNA-dependent protein kinase PKR. J. Virol. 81 182-92 PubMed GONUTS page
↑ ^5.0 ^5.1 Zhu, Y et al. (2012) Characterization of the RNA silencing suppression activity of the Ebola virus VP35 protein in plants and mammalian cells. J. Virol. 86 3038-49 PubMed GONUTS page
↑ Groseth, A et al. (2009) The Ebola virus ribonucleoprotein complex: a novel VP30-L interaction identified. Virus Res. 140 8-14 PubMed GONUTS page
↑ ^7.0 ^7.1 Huang, Y et al. (2002) The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein. Mol. Cell 10 307-16 PubMed GONUTS page
↑ ^8.0 ^8.1 Chang, TH et al. (2009) Ebola Zaire virus blocks type I interferon production by exploiting the host SUMO modification machinery. PLoS Pathog. 5 e1000493 PubMed GONUTS page
↑ ^9.0 ^9.1 ^9.2 Cárdenas, WB et al. (2006) Ebola virus VP35 protein binds double-stranded RNA and inhibits alpha/beta interferon production induced by RIG-I signaling. J. Virol. 80 5168-78 PubMed GONUTS page
↑ Zinzula, L et al. (2009) Purification and functional characterization of the full length recombinant Ebola virus VP35 protein expressed in E. coli. Protein Expr. Purif. 66 113-9 PubMed GONUTS page

[PMID:19828757-1] 1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 Jin, H et al. (2010) The VP35 protein of Ebola virus impairs dendritic cell maturation induced by virus and lipopolysaccharide. J. Gen. Virol. 91 352-61 PubMed GONUTS page

[PMID:21228243-2] 2.0 ^2.1 Fabozzi, G et al. (2011) Ebolavirus proteins suppress the effects of small interfering RNA by direct interaction with the mammalian RNA interference pathway. J. Virol. 85 2512-23 PubMed GONUTS page

[PMID:12829834-3] 3.0 ^3.1 ^3.2 ^3.3 Basler, CF et al. (2003) The Ebola virus VP35 protein inhibits activation of interferon regulatory factor 3. J. Virol. 77 7945-56 PubMed GONUTS page

[PMID:17065211-4] Feng, Z et al. (2007) The VP35 protein of Ebola virus inhibits the antiviral effect mediated by double-stranded RNA-dependent protein kinase PKR. J. Virol. 81 182-92 PubMed GONUTS page

[PMID:22238300-5] 5.0 ^5.1 Zhu, Y et al. (2012) Characterization of the RNA silencing suppression activity of the Ebola virus VP35 protein in plants and mammalian cells. J. Virol. 86 3038-49 PubMed GONUTS page

[PMID:19041915-6] Groseth, A et al. (2009) The Ebola virus ribonucleoprotein complex: a novel VP30-L interaction identified. Virus Res. 140 8-14 PubMed GONUTS page

[PMID:12191476-7] 7.0 ^7.1 Huang, Y et al. (2002) The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein. Mol. Cell 10 307-16 PubMed GONUTS page

[PMID:19557165-8] 8.0 ^8.1 Chang, TH et al. (2009) Ebola Zaire virus blocks type I interferon production by exploiting the host SUMO modification machinery. PLoS Pathog. 5 e1000493 PubMed GONUTS page

[PMID:16698997-9] 9.0 ^9.1 ^9.2 Cárdenas, WB et al. (2006) Ebola virus VP35 protein binds double-stranded RNA and inhibits alpha/beta interferon production induced by RIG-I signaling. J. Virol. 80 5168-78 PubMed GONUTS page

[PMID:19233284-10] Zinzula, L et al. (2009) Purification and functional characterization of the full length recombinant Ebola virus VP35 protein expressed in E. coli. Protein Expr. Purif. 66 113-9 PubMed GONUTS page

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EBOZM:VP35

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