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PMID:19041915
| Citation |
Groseth, A, Charton, JE, Sauerborn, M, Feldmann, F, Jones, SM, Hoenen, T and Feldmann, H (2009) The Ebola virus ribonucleoprotein complex: a novel VP30-L interaction identified. Virus Res. 140:8-14 |
|---|---|
| Abstract |
The ribonucleoprotein (RNP) complex of Ebola virus (EBOV) is known to be a multiprotein/RNA structure, however, knowledge is rather limited regarding the actual protein-protein interactions involved in its formation. Here we show that singularly expressed VP35 and VP30 are present throughout the cytoplasm, while NP forms prominent cytoplasmic inclusions and L forms smaller perinuclear inclusions. We could demonstrate the existence of NP-VP35, NP-VP30 and VP35-L interactions, similar to those described for Marburg virus (MARV) based on the redistribution of protein partners into NP and L inclusion bodies. Significantly, a novel VP30-L interaction was also identified and found to form as part of an NP-VP30-L bridge structure, similar to that formed by VP35. The identification of these interactions allows a preliminary model of the EBOV RNP complex structure to be proposed, and may provide insight into filovirus transcriptional regulation. |
| Links |
PubMed PMC3398801 Online version:10.1016/j.virusres.2008.10.017 |
| Keywords |
Animals; Cercopithecus aethiops; Ebolavirus/genetics; Ebolavirus/metabolism; Female; Fluorescent Antibody Technique; Gene Expression Regulation, Viral; Humans; Mice; Mice, Inbred BALB C; Nucleoproteins/genetics; Nucleoproteins/metabolism; Protein Interaction Mapping; Protein Structure, Quaternary; Transcription Factors/genetics; Transcription Factors/metabolism; Vero Cells; Viral Core Proteins/genetics; Viral Core Proteins/metabolism; Viral Proteins/genetics; Viral Proteins/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0030529: ribonucleoprotein complex |
ECO:0000314: |
C |
Figure 3: VP35 was coexpressed in a plasmid with known Ribonucleoprotein complex components NP and L. Antisera against all components were used in two-color co-immunofluorescence. This figure shows that VP35 was able to be co-localized with both known RNP components, forming a bridge. |
complete | ||||
| GO:0030529: ribonucleoprotein complex |
ECO:0000314: |
C |
Figure 3: VP30 was coexpressed in a plasmid with known Ribonucleoprotein complex components NP and L. Antisera against all components were used in two-color co-immunofluorescence. This figure shows that VP30 was able to be co-localized with both known RNP components, forming a bridge. |
complete | ||||
See also
References
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