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PMID:22730131
| Citation |
Courtney, CR, Cozy, LM and Kearns, DB (2012) Molecular characterization of the flagellar hook in Bacillus subtilis. J. Bacteriol. 194:4619-29 |
|---|---|
| Abstract |
The structure of the Gram-positive flagellum is poorly understood, and Bacillus subtilis encodes three proteins homologous to the flagellar hook protein from Salmonella enterica. Here we generated a modified B. subtilis hook protein that could be fluorescently stained using a cysteine-reactive dye. We used the fluorescently labeled hook to demonstrate that FlgE is the hook structural protein and that FliK regulated hook length. We further demonstrate that two proteins of unknown function, FlhO and FlhP, and the putative hook cap, FlgD, were required for hook assembly, such that when flhO, flhP, or flgD was mutated, hook protein was secreted into the supernatant. All mutants defective in hook completion resulted in homogeneously reduced σ(D)-dependent gene expression due to the action of the anti-sigma factor FlgM. |
| Links |
PubMed PMC3415477 Online version:10.1128/JB.00444-12 |
| Keywords |
Bacillus subtilis/genetics; Bacillus subtilis/metabolism; Bacillus subtilis/ultrastructure; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Flagella/genetics; Flagella/metabolism; Flagella/ultrastructure; Gene Expression Regulation, Bacterial; Promoter Regions, Genetic; Sigma Factor/genetics; Sigma Factor/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:0071978: bacterial-type flagellum-dependent swarming motility |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
| GO:0071978: bacterial-type flagellar swarming motility |
ECO:0000315: |
P |
Fig. 1C shows that a mutation in fliK abolished the swarming motility observed in WT cells. |
complete | ||||
| GO:0071978: bacterial-type flagellar swarming motility |
ECO:0000315: |
P |
Fig 1.C. Mutations were engineered into fLHO and expression of mutant reduced swarm motility. |
complete | ||||
|
involved_in |
GO:0071978: bacterial-type flagellum-dependent swarming motility |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
| GO:0043064: flagellum organization |
ECO:0000315: |
P |
Figures 5 A and B. FlgM shows higher miller unit activity compared to control |
complete | ||||
See also
References
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