PMID:14594839

Citation	McBride, MJ, Braun, TF and Brust, JL (2003) Flavobacterium johnsoniae GldH is a lipoprotein that is required for gliding motility and chitin utilization. J. Bacteriol. 185:6648-57
Abstract	Cells of Flavobacterium johnsoniae move rapidly over surfaces by gliding motility. The mechanism of this form of motility is not known. Six genes (gldA, gldB, gldD, gldF, gldG, and ftsX) that are required for gliding have been described. Tn4351 mutagenesis was used to identify another gene, gldH, which is required for cell movement. GldH mutants formed nonspreading colonies, and individual cells lacked the cell movements and ability to propel latex spheres along their surfaces that are characteristic of wild-type cells. gldH mutants also failed to digest chitin and were resistant to bacteriophages that infect wild-type cells. Introduction of pMM293, which carries wild-type gldH, restored to the gldH mutants colony spreading, cell motility, the ability to move latex spheres, phage sensitivity, and the ability to digest chitin. gldH encodes a predicted 141-amino-acid protein that localized to the membrane fraction. Labeling studies with [3H]palmitate demonstrated that GldH is a lipoprotein. GldB and GldD, which were previously described, also appear to be lipoproteins. GldH does not exhibit significant amino acid similarity to proteins of known function in the databases. Putative homologs of gldH of unknown function are found in motile (Cytophaga hutchinsonii) and apparently nonmotile (Bacteroides thetaiotaomicron, Bacteroides fragilis, Tannerella forsythensis, Porphyromonas gingivalis, and Prevotella intermedia) members of the Cytophaga-Flavobacterium-Bacteroides group.
Links	PubMed PMC262120
Keywords	Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Chitin/metabolism; DNA Transposable Elements; Flavobacterium/growth & development; Flavobacterium/metabolism; Flavobacterium/physiology; Gene Expression Regulation, Bacterial; Genetic Complementation Test; Lipoproteins/genetics; Lipoproteins/metabolism; Molecular Sequence Data; Movement; Mutagenesis, Insertional; Sequence Analysis, DNA
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
FLAJ1:GLDH	involved_in	GO:0006032: chitin catabolic process	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
FLAJ1:GLDH		GO:0071976: cell gliding	ECO:0000315:		P		Figure 2. Shows that wild type strain has gliding motility (A). Mutants do not (B). Mutants treated with plasmid containg GldH recover motility(C). Plasmids without GldH do not recover motility (D).	complete CACAO 7970
FLAJ1:GLDH		GO:0006032: chitin catabolic process	ECO:0000315:		P		Figure 5: Portion H-/+ Mutations in GldH show absence of chitinase activity, compared to when glDH mutants are co-expressed with plasmids containing GldH.	complete CACAO 7976
FLAJ1:GLDH	involved_in	GO:0071976: cell gliding	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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