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PMID:17617700
| Citation |
Basyuni, M, Oku, H, Tsujimoto, E and Baba, S (2007) Cloning and functional expression of cycloartenol synthases from mangrove species Rhizophora stylosa Griff. and Kandelia candel (L.) Druce. Biosci. Biotechnol. Biochem. 71:1788-92 |
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| Abstract |
To obtain cDNAs encoding oxidosqualene cyclase (OSC), we cloned two cDNAs, KcCAS and RsCAS, from roots of Kandelia candel (L.) Druce and leaves of Rhizophora stylosa Griff. by homology based PCR method respectively. The deduced amino acid sequences of both OSCs showed 82% homology to cycloartenol synthases from Lotus japonicus (OSC5) and Ricinus cummunis (RcCAS), suggesting that these are cycloartenol synthases of K. candel and R. stylosa. The genes obtained were expressed in a lanosterol synthase deficient Saccharomyces cerevisiae (ERG7) strain, GIL77. GC-MS analysis identified the accumulated reaction product in the yeast transformant to be cycloartenol, indicating that both KcCAS and RsCAS encode cycloartenol synthase. |
| Links | |
| Keywords |
Amino Acid Sequence; Cloning, Molecular; Intramolecular Transferases/biosynthesis; Intramolecular Transferases/genetics; Molecular Sequence Data; Rhizophoraceae/enzymology; Rhizophoraceae/genetics; Saccharomyces cerevisiae |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0016871: cycloartenol synthase activity |
ECO:0000314: |
F |
Figure 2: Shows that when the RsCAS gene was introduced into yeast, cycloartenols were produced |
complete | ||||
|
enables |
GO:0016871: cycloartenol synthase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
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