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PMID:9620975
| Citation |
Skarlatos, P and Dahl, MK (1998) The glucose kinase of Bacillus subtilis. J. Bacteriol. 180:3222-6 |
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| Abstract |
The open reading frame yqgR (now termed glcK), which had been sequenced as part of the genome project, encodes a glucose kinase of Bacillus subtilis. A 1.1-kb DNA fragment containing glcK complemented an Escherichia coli strain deficient in glucose kinase activity. Insertional mutagenesis of glcK resulted in a complete inactivation of glucose kinase activity in crude protein extracts, indicating that B. subtilis contains one major glucose kinase. The glcK gene encodes a 321-residue protein with a molecular mass of 33.5 kDa. The glucose kinase was overexpressed as a fusion protein to a six-His affinity tag and purified to homogeneity. The enzyme had K(m) values for ATP and glucose of 0.77 and 0.24 mM, respectively, and a Vmax of 93 mumol min-1 mg-1. A B. subtilis strain deficient for glucose kinase grew at the same rate on different carbon sources tested, including disaccharides such as maltose, trehalose, and sucrose. |
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| Keywords |
Bacillus subtilis/enzymology; Bacillus subtilis/genetics; Base Sequence; Chromosome Mapping; DNA Primers/genetics; DNA, Bacterial/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression; Genes, Bacterial; Genetic Complementation Test; Glucokinase/chemistry; Glucokinase/genetics; Glucokinase/metabolism; Kinetics; Molecular Sequence Data; Molecular Weight; Mutagenesis, Insertional; Open Reading Frames; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0004340: glucokinase activity |
ECO:0000315: |
F |
Table 2: Shows that a mutant strain MD186 (affecting the glcK gene) has complete loss of glucose kinase activity |
complete | ||||
|
enables |
GO:0004340: glucokinase activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
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