GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:12692346
| Citation |
Shalit, M, Guterman, I, Volpin, H, Bar, E, Tamari, T, Menda, N, Adam, Z, Zamir, D, Vainstein, A, Weiss, D, Pichersky, E and Lewinsohn, E (2003) Volatile ester formation in roses. Identification of an acetyl-coenzyme A. Geraniol/Citronellol acetyltransferase in developing rose petals. Plant Physiol. 131:1868-76 |
|---|---|
| Abstract |
The aroma of roses (Rosa hybrida) is due to more than 400 volatile compounds including terpenes, esters, and phenolic derivatives. 2-Phenylethyl acetate, cis-3-hexenyl acetate, geranyl acetate, and citronellyl acetate were identified as the main volatile esters emitted by the flowers of the scented rose var. "Fragrant Cloud." Cell-free extracts of petals acetylated several alcohols, utilizing acetyl-coenzyme A, to produce the corresponding acetate esters. Screening for genes similar to known plant alcohol acetyltransferases in a rose expressed sequence tag database yielded a cDNA (RhAAT1) encoding a protein with high similarity to several members of the BAHD family of acyltransferases. This cDNA was functionally expressed in Escherichia coli, and its gene product displayed acetyl-coenzyme A:geraniol acetyltransferase enzymatic activity in vitro. The RhAAT1 protein accepted other alcohols such as citronellol and 1-octanol as substrates, but 2-phenylethyl alcohol and cis-3-hexen-1-ol were poor substrates, suggesting that additional acetyltransferases are present in rose petals. The RhAAT1 protein is a polypeptide of 458 amino acids, with a calculated molecular mass of 51.8 kD, pI of 5.45, and is active as a monomer. The RhAAT1 gene was expressed exclusively in floral tissue with maximum transcript levels occurring at stage 4 of flower development, where scent emission is at its peak. |
| Links |
PubMed PMC166943 Online version:10.1104/pp.102.018572 |
| Keywords |
Acetyltransferases/genetics; Acetyltransferases/isolation & purification; Acetyltransferases/metabolism; Amino Acid Sequence; Esters/metabolism; Flowers/enzymology; Flowers/genetics; Flowers/growth & development; Flowers/metabolism; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Kinetics; Molecular Sequence Data; Molecular Structure; Monoterpenes/metabolism; Odors; Oils, Volatile/metabolism; RNA, Messenger/genetics; RNA, Messenger/metabolism; Rosa/enzymology; Rosa/genetics; Rosa/growth & development; Rosa/metabolism; Sequence Homology; Substrate Specificity; Terpenes/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0004026: alcohol O-acetyltransferase activity |
ECO:0000314: |
F |
Figure 4: Shows that the RhAAT1 gene product catalyzed the production of geranyl acetate from gerniol and aceyl-CoA and that this was dependent on the presence of the RhAAT1 gene product (enzyme). |
complete | ||||
|
enables |
GO:0004026: alcohol O-acetyltransferase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
See Help:References for how to manage references in GONUTS.
