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PMID:23290079
| Citation |
Griffin, LM, Cicchini, L and Pyeon, D (2013) Human papillomavirus infection is inhibited by host autophagy in primary human keratinocytes. Virology 437:12-9 |
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| Abstract |
Human papillomavirus (HPV) infection is severely limited in its natural host, primary human keratinocytes. Our data show HPV infectivity in primary keratinocytes is over 100- and 1,000-fold lower than in established keratinocyte cell lines NIKS and HaCaT, respectively. Here, we show that the basal level of autophagy in primary human foreskin keratinocytes (HFKs) is higher than in immortalized keratinocytes, and that HPV16 virions significantly induce autophagy in HFKs. Interestingly, HPV16 infectivity is dramatically enhanced by knockdown of essential autophagy genes as well as biochemical inhibition of autophagy. The increase in HPV16 infectivity by autophagy inhibition is most significant in HFKs, showing an inverse correlation with basal HPV16 infectivity in HFK, NIKS, HaCaT, and 293FT cells. Further, inhibition of autophagy delays degradation of HPV16 capsid proteins during virus trafficking, indicating that host autophagy induced by HPV16 virions inhibits infection of primary keratinocytes through rapid degradation of viral capsid proteins. |
| Links |
PubMed Online version:10.1016/j.virol.2012.12.004 |
| Keywords |
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| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0051607: defense response to virus |
ECO:0000315: |
P |
Fig 5 shows that ATG7 inhibits spread of HPV16 infection in primary keratinocytes. |
complete | ||||
| GO:0039521: suppression by virus of host autophagy |
ECO:0000315: |
P |
Fig 5 shows that the infection rate of HPV16 in primary keratinocytes is inhibited by ATG7 autophagy. |
complete | ||||
|
involved_in |
GO:0039521: suppression by virus of host autophagy |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0051607: defense response to virus |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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